Repository logo
 
Publication

Development of a sensitive ratiometric Imprinted hydrogel for the detection of matrix metalloproteinase 7 (MMP7) biomarker

2023Journal article Restricted access
Simple item page
dc.contributor.authorPiloto, Ana Margarida L.
dc.contributor.authorRibeiro, David S.M.
dc.contributor.authorSantos, João L.M.
dc.contributor.authorSales, Goreti
dc.date.accessioned2024-04-18T14:25:10Z
dc.date.available2024-04-18T14:25:10Z
dc.date.issued2023
dc.description.abstractA dual-emissive fluorescent probe was developed for the sensitive and selective detection of matrix metalloproteinase 7 (MMP7), a protein biomarker associated with pancreatic cancer. The ratiometric probe consisting of blue emitting carbon dots (CDs) and molecularly imprinted polymers (MIPs) previously assembled around red emitting quantum dots (QDs) was successfully combined for the detection of MMP7 protein. The concept is to use MIPs that function as the biorecognition elements, conjugated to cadmium telluride QDs, as the sensing system. The fluorescence intensity of red QDs is quenched with increasing concentrations of the analyte, acting as sensitive probes, while the fluorescence intensity of blue emitting CDs remains constant, acting as internal controls. The resultant fluorescence color changed from red to blue as a function of the MMP7 concentration, under a 365 nm UV lamp. The imprinted material MIP@QDs were successfully incorporated within a cellulose hydrogel containing CDs as reference probes. The resultant materials were designated as imprinted ratiometric hydrogels (imprinted rHGs). The fluorescence quenching of the imprinted rHGs occurred with increasing concentrations of MMP7, showing linearity in the range [1.49 × 10–11 – 1.92 × 10–9] g/mL, in 1000-fold diluted human serum. The imprinted rHGs showed an imprinting factor of 1.83 and a limit of detection of 4.11 × 10–12 g/mL. Overall, the imprinted rHGs developed in this work presented increased selectivity for the MMP7 protein over red emitting QDs nanoparticles and higher sensitivity comparatively to the non-imprinted rHGs.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationPiloto, A.M.L., Ribeiro, D.S.M., Santos, J.L.M., & Sales, G. (2024). Development of a sensitive ratiometric Imprinted hydrogel for the detection of matrix metalloproteinase 7 (MMP7) biomarker. ACS Appl. Opt. Mater. 2024, 2, 57−67. https://doi.org/10.1021/acsaom.3c00325pt_PT
dc.identifier.doi10.1021/acsaom.3c00325pt_PT
dc.identifier.issn2771-9855
dc.identifier.urihttp://hdl.handle.net/10400.22/25364
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relation.publisherversionhttps://pubs.acs.org/doi/10.1021/acsaom.3c00325pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/pt_PT
dc.subjectCellulosept_PT
dc.subjectMatrix metalloproteinase 7 (MMP7)pt_PT
dc.subjectPancreatic cancer (PC)pt_PT
dc.subjectQuantum dots (QDs)pt_PT
dc.subjectCarbon dots (CDs)pt_PT
dc.subjectMolecularly imprinted polymers (MIPs)pt_PT
dc.subjectDual-emission fluorescent probespt_PT
dc.titleDevelopment of a sensitive ratiometric Imprinted hydrogel for the detection of matrix metalloproteinase 7 (MMP7) biomarkerpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.citation.endPage67pt_PT
oaire.citation.issue1pt_PT
oaire.citation.startPage57pt_PT
oaire.citation.titleACS Applied Optical Materialspt_PT
oaire.citation.volume2pt_PT
person.familyNameL. Piloto Cerqueira
person.familyNameFerreira Sales
person.givenNameAna Margarida
person.givenNameMaria Goreti
person.identifier826970
person.identifier.ciencia-idAD1C-28E2-F781
person.identifier.ciencia-idE31F-1630-42BB
person.identifier.orcid0000-0002-2374-2467
person.identifier.orcid0000-0001-9936-7336
person.identifier.ridG-5788-2018
person.identifier.ridK-9199-2013
person.identifier.scopus-author-id8527885100
person.identifier.scopus-author-id7005174717
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationbed0bc46-bcdd-4f89-8eed-ac6c7620029e
relation.isAuthorOfPublicationb6d3a2d0-608a-4a92-834b-8d7b29069fef
relation.isAuthorOfPublication.latestForDiscoveryb6d3a2d0-608a-4a92-834b-8d7b29069fef

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
40_piloto-et-al-2023-development-of-a-sensitive-ratiometric-imprinted-hydrogel-for-the-detection-of-matrix.pdf
Size:
5.98 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

ISEP – CIETI – Artigos