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Employing bacteria machinery for antibiotic detection: Using DNA gyrase for ciprofloxacin detection

2021Journal article Embargoed access
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dc.contributor.authorCardoso, Ana Rita
dc.contributor.authorCarneiro, Liliana P.T.
dc.contributor.authorCabral-Miranda, Gustavo
dc.contributor.authorBachmann, Martin F.
dc.contributor.authorSales, Maria Goreti Ferreira
dc.date.accessioned2021-09-27T13:17:09Z
dc.date.embargo2031
dc.date.issued2021
dc.description.abstractThis work describes a new successful approach for designing biosensors that detect antibiotics. It makes use of a biomimetic strategy, by employing the biochemical target of a given antibiotic as its biorecognition element. This principle was tested herein for quinolones, which target DNA gyrase in bacteria. Ciprofloxacin (CIPRO) was tested as a representative antibiotic from the quinolone group; the sensitivity of biosensor to this group was confirmed by checking the response to another quinolone antibiotic (norfloxacin, NOR) and to a non-quinolone antibiotic (ampicillin, AMP). The biorecognition element used was DNA gyrase attached by ionic interactions to a carbon support, on a working electrode on common screen-printed electrodes (SPEs). The response against antibiotics was tested for increasing concentrations of CIPRO, NOR or AMP, and following the subsequent electrical changes by electrochemical impedance spectroscopy. The DNAgyrase biosensor showed sensitive responses for CIPRO and NOR, for concentrations down to 3.02 nM and 30.2 nM, respectively, with a very wide response range for CRIPRO, up to 30.2 µM. Its response was also confirmed selective for quinolones, when compared to its response against AMP. Further comparison to an immunosensor of similar design (adding antibodies instead of DNA gyrase) was made, revealing favourable features for the new biomimetic biosensor with 1.52 nM of limit of detection (LOD). Overall, the new approach presented herein is simple and effective for antibiotic detection, displaying a selective response against a given antibiotic group. The use of bacterial machinery as biorecognition element in biosensors may also provide a valuable tool to study the mechanism of action in bacterial cells of new drugs. This is especially important in the development of new drugs to fight bacterial resistance.pt_PT
dc.description.sponsorshipThe authors acknowledge funding from project PTDC/AAG-TEC/5400/2014 funded by European funds, through FEDER (European Funding or Regional Development) via COMPETE2020 – POCI (operational program for internationalization and competitively) and by national funding through the National Foundation for Science and Technology, I.P. (FCT). ARC also acknowledge funding to National Foundation for Science and Technology, I.P., through the PhD Grant, SFRH/BD/130107/2017.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.cej.2020.128135pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/18564
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relationalterado para: “NANOZYME+ / Driving nanozymes towards stereochemical recognition: application to biomolecules of interest in health”; NANOZYME+ / Driving nanozymes towards stereochemical recognition: application to Glucose sensing
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S1385894720342510?via%3Dihub#!pt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectBiochemical target of antibioticspt_PT
dc.subjectDNA gyrasept_PT
dc.subjectCiprofloxacinpt_PT
dc.subjectScreen-printed electrodespt_PT
dc.subjectElectrochemical biosensorpt_PT
dc.subjectAntibodiespt_PT
dc.titleEmploying bacteria machinery for antibiotic detection: Using DNA gyrase for ciprofloxacin detectionpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitlealterado para: “NANOZYME+ / Driving nanozymes towards stereochemical recognition: application to biomolecules of interest in health”; NANOZYME+ / Driving nanozymes towards stereochemical recognition: application to Glucose sensing
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/9471 - RIDTI/PTDC%2FAAG-TEC%2F5400%2F2014/PT
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/POR_NORTE/SFRH%2FBD%2F130107%2F2017/PT
oaire.citation.startPage128135pt_PT
oaire.citation.titleChemical Engineering Journalpt_PT
oaire.citation.volume409pt_PT
oaire.fundingStream9471 - RIDTI
oaire.fundingStreamPOR_NORTE
person.familyNameCardoso
person.familyNameFerreira Sales
person.givenNameAna Rita
person.givenNameMaria Goreti
person.identifier826970
person.identifier.ciencia-idF717-044B-1E8D
person.identifier.ciencia-idE31F-1630-42BB
person.identifier.orcid0000-0002-8641-0865
person.identifier.orcid0000-0001-9936-7336
person.identifier.ridK-9199-2013
person.identifier.scopus-author-id57125522400
person.identifier.scopus-author-id7005174717
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsembargoedAccesspt_PT
rcaap.typearticlept_PT
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relation.isAuthorOfPublication.latestForDiscoveryeeb95820-2553-4ad8-8d44-501ffd2c76ff
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