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Advisor(s)
Abstract(s)
O estudo e a monitorização da massa microbiótica marinha tem sido alvo de um crescente
interesse por parte de cientistas e biólogos marinhos. A análise desta matéria orgânica é
limitada pela capacidade de recolha de amostras de água e pelo acesso às zonas a analisar.
Esta tese procura uma solução que facilite a recolha destas amostras, mais especificamente,
amostras de DNA ambiental, e expanda a capacidade de acesso às zonas onde elas podem
ser recolhidas. São apresentadas quatro iterações de um sistema autónomo subaquático de
amostragem biológica, que, de uma iteração para a seguinte, procuram facilitar o processo
de recolha das amostras e, com a quarta iteração, aumentar leque de possíveis cenários de
aplicação, sendo este projetado para operar a profundidades até aos 90 metros. Os protótipos
também permitem a sua acoplação a veículos subaquáticos operados remotamente.
Esta tese apresenta o respetivo projeto e dimensionamento de cada um das iterações propostas.
Cada protótipo foi testado com a finalidade de validar o seu sistema hidráulico e
a respetiva integração com o sistema eletrónico, de forma a analisar a capacidade de dar
resposta aos objetivos a que cada um foi proposto. Posteriormente à validação dos sistemas
em laboratório, as primeiras três iterações foram implementadas com sucesso em cenário
real para a recolha de DNA ambiental.
The study of marine microbiome mass has been a subject of growing interest among marine scientists and biologists. The analysis of this organic matter is limited by the ability to collect water samples and access the areas of interest. This thesis seeks a solution that facilitates the collection of these kind of samples, specifically environmental DNA samples, offering a way to expand access to the areas where they can be collected. Four iterations of an autonomous underwater biological sampling system are presented, which, from one iteration to the next, aim to facilitate the sample collection process and, with the fourth iteration, increase the range of possible application scenarios, being designed to operate at depths of up to 90 meters. The prototypes also offer the possibility of being coupled to remotely operated underwater vehicles. For each proposed iteration of the system, the respective project and design is presented in this thesis. Each prototype was tested in order to validate its hydraulic system and its integration with the electronic system, so as to analyze its ability to meet the objectives for which each was proposed. After validating the systems in the laboratory, the first three iterations were successfully implemented in a real-life scenario to collect environmental DNA.
The study of marine microbiome mass has been a subject of growing interest among marine scientists and biologists. The analysis of this organic matter is limited by the ability to collect water samples and access the areas of interest. This thesis seeks a solution that facilitates the collection of these kind of samples, specifically environmental DNA samples, offering a way to expand access to the areas where they can be collected. Four iterations of an autonomous underwater biological sampling system are presented, which, from one iteration to the next, aim to facilitate the sample collection process and, with the fourth iteration, increase the range of possible application scenarios, being designed to operate at depths of up to 90 meters. The prototypes also offer the possibility of being coupled to remotely operated underwater vehicles. For each proposed iteration of the system, the respective project and design is presented in this thesis. Each prototype was tested in order to validate its hydraulic system and its integration with the electronic system, so as to analyze its ability to meet the objectives for which each was proposed. After validating the systems in the laboratory, the first three iterations were successfully implemented in a real-life scenario to collect environmental DNA.
Description
Keywords
Underwater Sampling Hydraulic system Environmental DNA Autonomous Omics Subaquático Ómica Amostragem Sistema hidráulico DNA ambiental Autónomo