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SERS and electrochemical impedance spectroscopy immunoassay for carcinoembryonic antigen

2021Journal article Restricted access
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dc.contributor.authorCastaño-Guerrero, Yuselis
dc.contributor.authorMoreira, Felismina
dc.contributor.authorSousa-Castillo, Ana
dc.contributor.authorCorrea-Duarte, Miguel A.
dc.contributor.authorSales, Goreti
dc.date.accessioned2021-09-27T13:57:47Z
dc.date.embargo2031-12
dc.date.issued2021
dc.description.abstractThis work describes an innovative dual detection approach, combining electrochemical and surface-enhanced Raman scattering (SERS) sequential readings, on the same sensing surface. This was achieved by establishing (i) an antibody binding stage on a suitably modified screen-printed electrode (Au-SPE), to produce an electrochemical signalling system, followed by (ii) a second antibody binding stage, on the same sensing surface, comprising gold nanostars (AuNS) with a suitable Raman reporter, and acting as a second signalling system (SERS). This simple principle is applied herein to carcinoembryonic antigen (CEA) detection. The first layer of antibodies was assembled on the Au-SPE previously modified with a cysteamine layer. Binding to CEA was allowed for 30 minutes. Electrochemical impedance spectroscopy (EIS) readings followed the several stages of Au-SPE modification and generated analytical data. After, the AuNS were modified with 4-aminothiophenol (4-ATP)/Ab-CEA, and incubated on the same sensing surface, to provide SERS data. The analytical features were checked for both EIS and SERS. In EIS, the sensor showed linear response range from 0.25 to 250 ng/mL, with a linear correlation coefficient of 0.991, evaluated in serum. It also demonstrated good selectivity against creatinine and glucose. Using the SERS as signalling system, the spectra confirmed differentiated signals from the background within 0.025 ng/mL to 250 ng/mL of CEA. As expected, the Raman signal of the reporter increased with increasing CEA concentrations, and contributed to confirm the accuracy of the analytical data. Overall, this approach is simple, and may be adaptable to new multiplexing devices, also being adaptable to mass production.pt_PT
dc.description.sponsorshipAuthors acknowledge funding to FEDER/COMPETE 2020 and Fundação para a Ciência e Tecnologia through the projects OligoFIT (ERA-NET Cofund, JPCOFUND2/0001/2019), RamSERS (NORTE-01-0247-FEDER-017834, call no. 33/SI/2015) and STRIP2SENSE (NORTE-01-0145-FEDER-024358-SAICT-POL/24358/2016). MINECO-Spain CTM2017-84050-R and Xunta de Galicia (IN607A 2018/5 and Centro Singular de Investigación de Galicia, Acc. 2016-2019) are also acknowledged. YCG also acknowledge the PhD grant reference SFRH/BD/145590/2019 to Fundação para a Ciência e Tecnologia.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.doi10.1016/j.electacta.2020.137377pt_PT
dc.identifier.issn0013-4686
dc.identifier.urihttp://hdl.handle.net/10400.22/18568
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relationNORTE-01-0247-FEDER-017834pt_PT
dc.relationNORTE-01-0145-FEDER-024358-SAICT-POL/24358/2016pt_PT
dc.relationSERS-based biosensors for on-site determination of pancreatic cancer biomarkers
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0013468620317709?via%3Dihubpt_PT
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/pt_PT
dc.subjectCarcinoembryonic Antigenpt_PT
dc.subjectImmunosensorpt_PT
dc.subjectElectrochemical impedance spectroscopypt_PT
dc.subjectScreen-printed electrodept_PT
dc.subjectSurface-enhanced Raman spectroscopypt_PT
dc.titleSERS and electrochemical impedance spectroscopy immunoassay for carcinoembryonic antigenpt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitleSERS-based biosensors for on-site determination of pancreatic cancer biomarkers
oaire.awardURIinfo:eu-repo/grantAgreement/FCT//SFRH%2FBD%2F145590%2F2019/PT
oaire.citation.startPage137377pt_PT
oaire.citation.titleElectrochimica Actapt_PT
oaire.citation.volume366pt_PT
person.familyNameMoreira
person.familyNameFerreira Sales
person.givenNameFelismina
person.givenNameMaria Goreti
person.identifier1589429
person.identifier826970
person.identifier.ciencia-idC917-6A46-A270
person.identifier.ciencia-idE31F-1630-42BB
person.identifier.orcid0000-0003-4237-8952
person.identifier.orcid0000-0001-9936-7336
person.identifier.ridK-9199-2013
person.identifier.scopus-author-id23486193000
person.identifier.scopus-author-id7005174717
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsrestrictedAccesspt_PT
rcaap.typearticlept_PT
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