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Responses of the alga Pseudokirchneriella subcapitata to long-term exposure to metal stress

2015Journal article Restricted access
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dc.contributor.authorMachado, Manuela D.
dc.contributor.authorLopes, Ana R.
dc.contributor.authorSoares, Eduardo V.
dc.date.accessioned2016-01-15T10:27:05Z
dc.date.available2016-01-15T10:27:05Z
dc.date.issued2015
dc.description.abstractThe green alga Pseudokirchneriella subcapitata has been widely used in ecological risk assessment, usually based on the impact of the toxicants in the alga growth. However, the physiological causes that lead algal growth inhibition are not completely understood. This work aimed to evaluate the biochemical and structural modifications in P. subcapitata after exposure, for 72 h, to three nominal concentrations of Cd(II), Cr(VI), Cu(II) and Zn(II), corresponding approximately to 72 h-EC10 and 72 h-EC50 values and a high concentration (above 72 h-EC90 values). The incubation of algal cells with the highest concentration of Cd(II), Cr(VI) or Cu(II) resulted in a loss of membrane integrity of ~16, 38 and 55%, respectively. For all metals tested, an inhibition of esterase activity, in a dose-dependent manner, was observed. Reduction of chlorophyll a content, decrease of maximum quantum yield of photosystem II and modification of mitochondrial membrane potential was also verified. In conclusion, the exposure of P. subcapitata to metals resulted in a perturbation of the cell physiological status. Principal component analysis revealed that the impairment of esterase activity combined with the reduction of chlorophyll a content were related with the inhibition of growth caused by a prolonged exposure to the heavy metals.pt_PT
dc.identifier.doi10.1016/j.jhazmat.2015.04.022pt_PT
dc.identifier.urihttp://hdl.handle.net/10400.22/7400
dc.language.isoengpt_PT
dc.peerreviewedyespt_PT
dc.publisherElsevierpt_PT
dc.relationNORTE-07-0124-FEDER-000028pt_PT
dc.relation2013 - Strategic Project
dc.relation.ispartofseriesJournal of Hazardous Materials;Vol. 296
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0304389415003040pt_PT
dc.subjectEsterase activitypt_PT
dc.subjectHeavy metals toxicitypt_PT
dc.subjectMembrane integritypt_PT
dc.subjectMitochondrial membrane potentialpt_PT
dc.subjectPhotosynthetic activitypt_PT
dc.titleResponses of the alga Pseudokirchneriella subcapitata to long-term exposure to metal stresspt_PT
dc.typejournal article
dspace.entity.typePublication
oaire.awardTitle2013 - Strategic Project
oaire.awardURIinfo:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/PEst-OE%2FEQB%2FLA0023%2F2013/PT
oaire.citation.endPage92pt_PT
oaire.citation.startPage82pt_PT
oaire.citation.titleJournal of Hazardous Materialspt_PT
oaire.citation.volume296pt_PT
oaire.fundingStream6817 - DCRRNI ID
person.familyNameSoares
person.givenNameEduardo
person.identifier93637
person.identifier.ciencia-idD612-0D0E-4984
person.identifier.orcid0000-0003-2280-5291
person.identifier.ridO-5908-2015
person.identifier.scopus-author-id7102060173
project.funder.identifierhttp://doi.org/10.13039/501100001871
project.funder.nameFundação para a Ciência e a Tecnologia
rcaap.rightsclosedAccesspt_PT
rcaap.typearticlept_PT
relation.isAuthorOfPublicationb3ebdee1-84fa-4183-a1d8-c3cdcaaef219
relation.isAuthorOfPublication.latestForDiscoveryb3ebdee1-84fa-4183-a1d8-c3cdcaaef219
relation.isProjectOfPublication8a6913fb-272b-4c7d-91e3-7f3a5bdf1f39
relation.isProjectOfPublication.latestForDiscovery8a6913fb-272b-4c7d-91e3-7f3a5bdf1f39

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