Publication
An electrochemically synthesized molecularly imprinted polymer for highly selective detection of breast cancer biomarker CA 15-3: a promising point-of-care biosensor
| datacite.subject.fos | Ciências Naturais::Ciências Biológicas | |
| datacite.subject.sdg | 03:Saúde de Qualidade | |
| dc.contributor.author | Oliveira, Daniela | |
| dc.contributor.author | Romaguera Barcelay, Yonny | |
| dc.contributor.author | Moreira, Felismina | |
| dc.date.accessioned | 2025-04-16T18:04:51Z | |
| dc.date.available | 2025-04-16T18:04:51Z | |
| dc.date.issued | 2024-05 | |
| dc.description.abstract | In this study, a molecularly imprinted polymer film (MIP) was prepared on the surface of a disposable carbon screen-printed electrode (C-SPE) using (3-acrylamidopropyl)trimethylammonium chloride (AMPTMA) as a functional monomer and the cancer biomarker carbohydrate antigen 15-3 (CA 15-3) as a template. The MIP was synthesized by in situ electropolymerization (ELP) of the AMPTMA monomer in the presence of the CA 15-3 protein on the C-SPE surface. The target was subsequently removed from the polymer matrix by the action of proteinase K, resulting in imprinted cavities with a high affinity for CA 15-3. Electrochemical techniques such as cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) were used to characterize the different phases of the sensor assembly. Chemical and morphological analysis was performed using RAMAN and scanning electron microscopy (SEM). CA 15-3 was successfully detected in a wide working range from 0.001 U mL−1 to 100 U mL−1 with a correlation coefficient (R2) of 0.994 in 20 min. The MIP sensor showed minimal interference with other cancer proteins (CEA and CA 125). Overall, the developed device provides a rapid, sensitive, and cost-effective response in the detection of CA 15-3. Importantly, this comprehensive approach appears suitable for point-of-care (PoC) use, particularly in a clinical context. | eng |
| dc.description.sponsorship | The authors acknowledge the financial support from the IBEROS + project (Instituto de Biofabricación en Red para el Envejecimiento Saludable), INTERREG POCTEP/0072_IBEROS_MAIS_1_E within the cooperation region of Galicia/Spain and North of Portugal. Additionally, they would like to thank the partial support from the Portuguese Foundation for Science and Technology (FCT), through grants UIDB/04730/2020 and UIDP/04730/2020. Daniela O. acknowledges funding from the Portuguese Foundation for Science and Technology (FCT), I. P., through the PhD grant reference SFRH/BD/137832/2018. | |
| dc.identifier.citation | Daniela Oliveira, Yonny Romaguera Barcelay, Felismina T. C. Moreira An electrochemically synthesized molecularly imprinted polymer for highly selective detection of breast cancer biomarker CA 15-3: a promising point-of-care biosensor. RSC Adv., 2024,14, 15347-15357. doi: 10.1039/D4RA02051K | |
| dc.identifier.doi | 10.1039/D4RA02051K | |
| dc.identifier.issn | 2046-2069 | |
| dc.identifier.uri | http://hdl.handle.net/10400.22/29983 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | Royal Society of Chemistry | |
| dc.relation | Center for Innovation in Industrial Engineering and Technology | |
| dc.relation | Center for Innovation in Industrial Engineering and Technology | |
| dc.relation.hasversion | https://pubs.rsc.org/en/content/articlehtml/2024/ra/d4ra02051k | |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.title | An electrochemically synthesized molecularly imprinted polymer for highly selective detection of breast cancer biomarker CA 15-3: a promising point-of-care biosensor | eng |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Center for Innovation in Industrial Engineering and Technology | |
| oaire.awardTitle | Center for Innovation in Industrial Engineering and Technology | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04730%2F2020/PT | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDP%2F04730%2F2020/PT | |
| oaire.citation.endPage | 15357 | |
| oaire.citation.startPage | 15347 | |
| oaire.citation.title | RSC Advances | |
| oaire.citation.volume | 14 | |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |
| person.familyName | Romaguera Barcelay | |
| person.familyName | Moreira | |
| person.givenName | Yonny | |
| person.givenName | Felismina | |
| person.identifier | 1589429 | |
| person.identifier.ciencia-id | A01F-6369-FA9F | |
| person.identifier.ciencia-id | C917-6A46-A270 | |
| person.identifier.orcid | 0000-0002-8091-1212 | |
| person.identifier.orcid | 0000-0003-4237-8952 | |
| person.identifier.scopus-author-id | 23486193000 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
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