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- Development of a method based on HPLC-DAD for the detection and quantification of glutamate and gamma-aminobutyric acidPublication . Machado, Patrícia; Teixeira, Dulce; Vieira, Mónica; Prudêncio, CristinaWith the increasing incidence of neurodegenerative diseases, the need arose to study several molecules that may be useful to the study of new therapies and to treat the symptoms that come with these diseases, as is the case of depression. GABA and glutamate have a very important role in the homeostasis of the organism and molecules of interest and potential from the point of view of diagnosis are revealed. HPLC is a method that allows the detection and quantification of various analytes in different biological matrices, allowing rapid results with high precision, sensitivity and specificity.
- Development of a new method for the detection and quantification of octopamine and 3-nitrotyrosinePublication . Teixeira, Dulce; Prudêncio, Cristina; Azenha, Manuel; Vieira, MónicaNeurodegenerative diseases affect around 30 million people worldwide, thus new methods that can facilitate research of such diseases are essential. Neurotransmitters, characterised as critical regulators of several neurological disorders, have been the target for the development of such methods. Besides, oxidative/ nitrosative stress has been associated with several neurodegenerative diseases and is thought to play an important role in their pathogenesis. Therefore, the development of bioanalytical methods for quantification of neurotransmitters and oxidative stress-associated molecules seems to be an interesting approach. The aim of this study was to develop a simple, rapid, low-cost and sensitive octopamine (OCT) and 3-nitrotyrosine (3-NT) quantification method for use in biological models.