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Browsing by Author "Rufino Arcanjo, Daniel Dias"

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  • Copper nanoparticles stabilized with cashew gum: Antimicrobial activity and cytotoxicity against 4T1 mouse mammary tumor cell line
    Publication . Amorim, Adriany; Mafud, Ana Carolina; Nogueira, Silvania; Ramos-Jesus, Joilson; Araújo, Alyne Rodrigues de; Plácido, Alexandra; Neta, Maria Brito; Alves, Michel Muálem Moraes; Carvalho, Fernando Aécio Amorim; Rufino Arcanjo, Daniel Dias; Braun, Sacha; López, Marta Sánchez-Paniagua; López-Ruiz, Beatriz; Delerue-Matos, Cristina; Mascarenhas, Yvonne; Silva, Durcilene; Eaton, Peter; Leite, José Roberto Souza Almeida
    Copper nanoparticles stabilized with cashew (CG-CuNPs) were synthesized by reduction reaction using ascorbic acid and sodium borohydride, using the cashew gum (CG) as a natural polymer stabilizer. Dynamic light scattering, atomic force microscopy, Fourier-transform infrared spectroscopy, UV-Vis spectrophotometry, and x-ray diffraction were used to characterize the nanoparticles (CG-CuNPs), and copper was quantified by electrochemical measurement. The UV-vis spectra of the CG-CuNPs confirmed the formation of nanoparticles by appearance of a surface plasmon band at 580 nm after 24 h of reaction. The Fourier-transform infrared spectrum of CG-CuNPs showed the peak at 1704 cm−1 from cashew gum, confirming the presence of the gum in the nanoparticles. The average size of CG-CuNPs by dynamic light scattering and atomic force microscopy was around 10 nm, indicating small, approximately spherical particles. Antimicrobial assays showed that CG-CuNPs had activity against Staphylococcus aureus ATCC 29213 with a minimal inhibitory concentration of 0.64 mM. The cytotoxicity assay on BALB/c murine macrophages showed lower cytotoxic effects for CG-CuNPs than CuSO4·5H2O. Viability cell assays for CG-CuNPs at (0.250 mM) inhibited by 70% the growth of 4T1 LUC (4T1 mouse mammary tumor cell line) and NIH 3T3 cells (murine fibroblast cells) over a 24-h period. Therefore, CG-CuNPs can be used as an antimicrobial agent with lower cytotoxic effects than the CuSO4·5H2O precursor.
    2019-08-01Journal article Open access Show more