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Browsing by Author "Oliveira, Sofia Costa de"

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  • Flow cytometric antimicrobial susceptibility test for gram-negative bacilli directly from positives urines
    Publication . Oliveira, Joana Santos; Santos, Rita Teixeira; Oliveira, Sofia Costa de; Dias, Ana Silva; Oliveira, Inês Martins; Rodrigues, Acácio G.; Vaz, Cidália Pina
    Urinary tract infections (UTIs) are the most frequent infections in both hospitalized and community patients. These infections may provoke serious complications especially in children, pregnant human and elderly. The rate of recurrent UTIs is high and the increasing number of resistant bacteria is intensified by incorrect or excessive use of antimicrobial drugs. Consequently, rapid antimicrobial susceptibility tests are urgent. FASTinov® kits allow the determination of antimicrobial susceptibility profile directly from biological products (time-to-result, TTR, 2h). In this work, we evaluated the performance of FASTinov® gramneg kit for AST on gram-negative bacilli directly from urine.
    2018-05-18Conference object Open access Show more
  • Screening of positive urines using Sysmex UF-1000i flow cytometer and identification by MALDI-TOF mass spectrometer
    Publication . Oliveira, Joana Santos; Santos, Rita Teixeira; Oliveira, Sofia Costa de; Dias, Ana Silva; Oliveira, Inês Martins; Rodrigues, Acácio G.; Vaz, Cidália Pina
    Urinary tract infections (UTIs) are the most common infections both in hospitalized and community patients often leading to severe complications. Gram-negative bacilli are the most prevalent microorganisms often resistant to main antimicrobial agents. Microbiology diagnosis is necessary but take at least 48 hours to provide results. Sysmex UF-1000i is an automated urine flow cytometer that have been described as able to avoid the culture on negative samples. We evaluated this equipment in order to screen positive urines with gram-negative bacilli >1 x 105/mL comparing it with the routine method. Additionally, we have developed an extraction protocol for identification of the strains directly from urine on MALDITOF.
    2018-05-18Conference object Open access Show more