Browsing by Author "Faria, Miguel A."
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- Bioaccessibility and intestinal uptake of minerals from different types of home-cooked and ready-to-eat beansPublication . Faria, Miguel A.; Araújo, Alfredo; Pinto, Edgar; Oliveira, César; Oliva-Teles, MT; Almeida, Agostinho; Delerue-Matos, Cristina; Ferreira, Isabel M.P.L.V.O.Limited information exists on the bioaccessibility and intestinal uptake of essential minerals from different types of beans cooked through different cooking methods. This study aimed to estimate the in vitro bioaccessibility and intestinal uptake of the essential minerals K, Ca, Mg, Fe, Zn, Mn, Cu and Mo from four different types of beans (kidney, black, white and cowpea) cooked under different conditions (home-cooked and industrially processed canned product). Results showed that the bioaccessibility and uptake of most essential minerals is higher in canned beans. Mn was the mineral that presented the highest bioaccessibility (43–63%), and Mo had the lowest (3–36%). The highest uptake was observed for Mg (59.9%), while the lowest was found for Fe (10.5%). Regarding the type of beans, white beans presented the highest bioaccessibility and uptake for all the analyzed essential minerals and thus its consumption is preferable from a nutritional point of view.
- Effect of skimmed milk on intestinal tract: prevention of increased reactive oxygen species and nitric oxide formationPublication . Pinho, Susana C. M.; Faria, Miguel A.; Melo, Armindo; Pinto, Edgar; Almeida, Agostinho; Alves, Rui; Cabrita, Ana R. J.; Fonseca, António J. M.; Ferreira, Isabel M. P. V. O.The capacity of skimmed milk to neutralise increased reactive oxygen species (ROS) and to attenuate nitric oxide (NO) production, as well as to present cytoprotective effect at the intestinal level was assessed after in vitro gastro-intestinal digestion. The impact on ROS modulation was evaluated at a non-cytotoxic concentration of hydrogen peroxide (H2O2) in a co-culture of Caco-2 and HT-29 intestinal cells. In parallel, a cytotoxic concentration of H2O2 was used to study the effect of digested milk against induced cell apoptosis. Concerning induced NO production, it was evaluated using the model lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. Results showed that digested milk prevented the increase of basal ROS level in the intestinal epithelium and attenuated NO production by LPS-stimulated macrophage cells. In the H2O2-induced cytotoxicity assay, digested milk had no protection against apoptosis, confirmed by the failure in attenuating activated caspase-3/7.