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Percorrer por autor "Dias, Bruna"

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  • Does iron and B12 Supplementation play a role in diet-induced changes in muscle mass? – Findings from the veggienutri cross-sectional study
    Publication . Pinheiro, Cátia; Silva, Flávia; Rocha, Inês; Martins, Carina; Giesteira, Liliana; Dias, Bruna; Lucas, Ana; Alexandre, Ana Margarida; Ferreira, Catarina; Viegas, Bruna; Bracchi, Isabella; Guimarães, Juliana; Amaro, Joana; Amaral, Teresa F.; Dia, Cláudia Camila; Oliveira, Andreia; Ndrio, Altin; Guimarães, João Tiago; Leite, João Costa; Negrão, Rita; Keating, Elisa
    In Portugal, lack of information regarding vegetarian populations and difficult access to nutritional counselling may exacerbate the risks of poorly planned plant-based diets. To study body composition differences between vegetarian and omnivorous adults, and to identify putative underlying nutritional and health parameters. 425 omnivorous (OMNI), lacto-ovo-vegetarian (LOV) or vegan (VEG) adults living in Portugal free of any chronic disease were included. Participants answered a food frequency and a sociodemographic and lifestyle questionnaire. Bioelectric impedance analysis was performed, and fasting blood samples were collected for health biomarkers analysis. Total protein intake was significantly lower for stricter vegetarian habits (median (P25; P75) in g/day: 98.6 (79.5; 123.1), 90.4 (65.9; 121.0), and 87.6 (59.8; 118.5) for OMNI, LOV and VEG, respectively; p = 0.020). Compared to being OMNI, being LOV, but not VEG, was independently associated with having +4.8 % (p = 0.002) fat mass and −2.2 % (p = 0.043) muscle mass (model adjusted for sex, age, marital status, physical exercise, multivitamin supplement intake and nutritional counselling). On the other hand, isolated B12 supplements were most used by VEG (93% in VEG vs. 17% in OMNI and 59% in LOV, p = 0.001), while isolated iron supplements were most used by LOV (29% in LOV vs. 14% in OMNI and 13% in VEG, p = 0.042). Among VEG, B12 blood levels correlated negatively with blood homocysteine (rs = −0.386, p < 0.001) and positively with % muscle mass (rs = 0.136, p = 0.005) and iron supplement users presented higher C-reactive protein (p = 0.014) and lower % muscle mass (p = 0.003), when compared to non-users. Our data suggest that among VEG, B12 supplementation may rescue from low-protein-induced muscle mass loss, while among LOV, ironrelated inflammation may exacerbate it.
    2025-05Comunicação em conferência Acesso aberto Ver mais
  • Proteomics characterization of human uterine samples during reproductive ageing
    Publication . Dias, Bruna; Formoso, Inês; Guedes-Martins, Luís; Almeida, Henrique; Silva, Elisabete; Silva, André M.N.; Matos, Liliana
    Nowadays, postponing motherhood has become more common in modern societies. Such decision rises major social and health concerns, as advanced maternal age associates with low fertility, pregnancy complications and a greater need for assisted reproductive techniques. Female reproductive ageing is associated with alterations that are particularly evident in the ovaries. Yet, given the pivotal contribution of the uterus to embryo adhesion, implantation, and fetal development, we believe that age-dependent uterine changes impair fertility. Evidence in mice already reported age-associated uterine changes. Also, albumin carbonylation was found increased in uterine samples from older term-pregnant women. Moreover, preliminary data show changes in composition and oxidation status of uterine extracellular matrix proteins with higher maternal age. We hypothesize that the uterine proteome changes during reproductive ageing, impairing tissue structure and function. This study aims to identify age-related alterations in uterine protein composition through a complete proteomics approach. Uterine samples were collected during c-section from termpregnant women and immediately fixed for paraffin-embedding or frozen for molecular analysis. Six samples were selected: three from younger and three from reproductively aged women. Paraffin-embedded samples were histologically analyzed after H+E staining. Protein lysates were obtained after sample homogenization and total protein content was determined and were then separated by SDS-PAGE, stained with Coomassie Blue, and will be subjected to a MS based proteomics study. Histological analysis of the samples has proven their uterine origin. SDS-PAGE results confirmed the successful protein extraction. The identification of uterine proteins differentially expressed according to maternal age will allow the development of therapeutic approaches, aiming to attenuate age-associated female fertility decline.
    2024-05Póster em conferência Acesso aberto Ver mais