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Percorrer por autor "Almeida, Nuno"

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  • Evaluation of the concordance of pharmacogenomic information in the RCM and FI with the international recommendations of CPIC and PharmGKB
    Publication . Almeida, Nuno; Vasconcelos, Ana; Lacerda, Ivo; Santos, Marlene; Santos, Marlene
    Pharmacogenomics plays an increas-ing role in optimizing antiviral therapy, allowing the choice and dosage of drugs to be tailored to the pa-tient’s genetic profile. However, the integration of this information into regulatory documents, such as the Summary of Product Characteristics (SmPC) and the Patient Information Leaflet (PIL), remains inconsistent. To evaluate the degree of concordance between the pharmacogenomic information present-ed in the SmPC and PIL of antiviral drugs with the international recommendations of the Clinical Phar-macogenetics Implementation Consortium (CPIC) and the evidence levels reported in PharmGKB. An analysis was conducted on a set of 146 marketed antiviral drugs, assessing the presence of pharmacogenomic recommendations in their respec-tive SmPCs and PILs. These data were compared with CPIC guidelines and the pharmacogenomic evidence levels assigned by PharmGKB. Results: Only 14 out of the 146 drugs analysed pre-sented recommendations based on CPIC. Regarding PharmGKB evidence levels, most active substanc-es exhibited level 3. Complete agreement with CPIC guidelines was observed in only 2 SmPCs, while other 5 did not consider any genetic polymorphisms. The re-maining documents contained incomplete or partially aligned pharmacogenomic information relative to in-ternational recommendations. The results show that, with the excep-tion of abacavir, there is a discrepancy between the available pharmacogenomic evidence and its inte-gration into the SmPCs of antiviral medicines. This misalignment reinforces the need for more compre-hensive studies and effective harmonization between international guidelines and the content of SmPCs, promoting the adoption of personalized medicine.
    2026-05-29Documento de conferência Acesso aberto Ver mais
  • How electric fields may shape inhibition: A molecular dynamics study of GABA-Receptors
    Publication . Almeida, Nuno; Sousa, Nuno; Vieira, Mónica; Rocha, Nuno; Almeida Vieira, Mónica Andreia
    Transcranial direct current stimulation (tDCS) is a non-invasive technique used to treat psychiatric and neurological disorders by modulating brain activity using electrical currents. Although effective the mechanisms by which tDCS alters brain function. particularly its effect on proteins such as GABA-A receptors, are still unclear. GABA-A receptors regulate neuronal excitability by allowing chloriele ions to enter the cells. leading to inhibitory effects. It is assumed that tDCS alters the conformation and dynamics of these receptors and thus impairs their function. To investig te these changes, molecular dynamics simulations could be used to model how electric fields affect GABA-A receptors embedded in a bilipid membrane. The hypothesis is that tDCS causes rapid, transient conformational changes that influence the duration of ion channel opening and thus modulate chloriele entry. The bilipiel membrane also plays a crucial role, as its interaction with the receptor can enhance or attenuate the tDCS effects. Due to the complexity of the system, high-performance computing resources are essential to capture dynamic changes at atomic resolution over biologically relevant timescales. Although the results are not yet available at the time of this submission, this work-in-progress poster will present the simulation strategy, system setup, and computational protocols employed. By sharing our methodological approach, we aim to foster discussion and receive early feedback from the scientific community, which will inform subsequent data analysis and interpretation.
    2026Póster em conferência Acesso aberto Ver mais