Publication
Paper-based biomimetic test-strip for CA15-3 with coloured readout
| datacite.subject.fos | Engenharia e Tecnologia | |
| datacite.subject.sdg | 09:Indústria, Inovação e Infraestruturas | |
| dc.contributor.author | Carneiro, Mariana C. C. G. | |
| dc.contributor.author | Rodrigues, Lígia R. | |
| dc.contributor.author | Moreira, Felismina | |
| dc.contributor.author | Sales, M. Goreti F. | |
| dc.date.accessioned | 2025-02-11T19:13:01Z | |
| dc.date.available | 2025-02-11T19:13:01Z | |
| dc.date.issued | 2024 | |
| dc.description.abstract | The combination of molecularly-imprinted polymers (MIPs) on paper substrates for capturing a cancer antigen 15–3 (CA15-3) with the traditional coloured transduction scheme of enzyme-linked immunosorbent assay (ELISA) using 3,3′,5,5′-tetramethylbenzidine (TMB), horseradish peroxidase (HRP) and H2O2 is presented here for the first time. Here, the paper surface was modified with a silane derivative containing an amine function that allows subsequent binding of 3-aminophenylboronic acid (3-APBA). The target protein, CA15-3, was then bound via the boronic groups of APBA. The empty space around CA15-3 was filled by polymerization of dopamine. Finally, the CA15-3 template was removed by breaking the imine function to create vacant sites for which CA15-3 has a high affinity. Binding of CA15-3 was detected by oxidation of TMB substrate by HRP in the presence of H2O2. The results showed that the MIP was able to selectively recognize CA15-3 within 3–500 U mL−1, even in complex samples. Quantitative data were extracted by analysing the colour coordinates of images captured with a smartphone using ImageJ. This selective behaviour was confirmed by comparison with a control material (non– polymer, NIP). Overall, the described approach has advantages over conventional ELISA, namely low cost and high stability, which is due to the use of MIP as a trapping element acting as a selective pre-concentration point of CA15-3. To our knowledge, this is the first biomimetic ELISA (B-ELISA) on paper substrate used for macromolecules such as proteins. We believe that this approach has the potential to be applied to other protein disease biomarkers, making it a suitable tool for screening glycoproteins at the point-of-care. | eng |
| dc.description.sponsorship | This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit. Authors also acknowledge FCT for funding the PhD grant reference SFRH/BD/131959/2017. | |
| dc.identifier.citation | Carneiro, M. C.C.G., Rodrigues, L. R., Moreira, F., & Sales, M. G. F. (2024). Paper-based biomimetic test-strip for CA15-3 with coloured readout. Microchemical Journal, 196. https://doi.org/10.1016/j.microc.2023.109640. | |
| dc.identifier.doi | 10.1016/j.microc.2023.109640 | |
| dc.identifier.issn | 0026-265X | |
| dc.identifier.uri | http://hdl.handle.net/10400.22/29471 | |
| dc.language.iso | eng | |
| dc.peerreviewed | yes | |
| dc.publisher | Elsevier | |
| dc.relation | Centre of Biological Engineering of the University of Minho | |
| dc.relation.hasversion | https://www.sciencedirect.com/science/article/pii/S0026265X23012596 | |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | |
| dc.subject | Molecularly imprinted polymer | |
| dc.subject | Biomimetic enzyme-linked immunosorbent assay | |
| dc.subject | Point-of-care | |
| dc.subject | Cellulose paper | |
| dc.subject | Cancer antigen 15–3 | |
| dc.subject | Protein biomarker | |
| dc.title | Paper-based biomimetic test-strip for CA15-3 with coloured readout | eng |
| dc.type | journal article | |
| dspace.entity.type | Publication | |
| oaire.awardTitle | Centre of Biological Engineering of the University of Minho | |
| oaire.awardURI | info:eu-repo/grantAgreement/FCT/6817 - DCRRNI ID/UIDB%2F04469%2F2020/PT | |
| oaire.citation.title | Microchemical Journal | |
| oaire.citation.volume | 196 | |
| oaire.fundingStream | 6817 - DCRRNI ID | |
| oaire.version | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |
| project.funder.identifier | http://doi.org/10.13039/501100001871 | |
| project.funder.name | Fundação para a Ciência e a Tecnologia | |
| relation.isProjectOfPublication | 222bb02c-0380-41b1-8083-7c6f585e2db3 | |
| relation.isProjectOfPublication.latestForDiscovery | 222bb02c-0380-41b1-8083-7c6f585e2db3 |