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Development of electrochemical genosensors for the CYPC*2 gene polymorphism detection

2022-05Conference object Open access
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dc.contributor.authorSousa, António J. S. F.
dc.contributor.authorCosta, Inês
dc.contributor.authorBanegas, Rodrigo S.
dc.contributor.authorMorais, Stephanie L.
dc.contributor.authorMagalhães, Júlia
dc.contributor.authorRodrigues, Valentina
dc.contributor.authorDelerue-Matos, Cristina
dc.contributor.authorRamos-Jesus, Joilson
dc.contributor.authorFerreira-Fernandes, Hygor
dc.contributor.authorPinto, Giovanny R.
dc.contributor.authorSantos, Marlene
dc.contributor.authorBarroso, M. Fátima
dc.date.accessioned2023-09-14T15:23:39Z
dc.date.available2023-09-14T15:23:39Z
dc.date.issued2022-05
dc.description.abstractPharmacogenetic studies search for heritable genetic polymorphisms that influence responses to drug therapy. Pharmacogenetics has many possible applications in cardiovascular pharmacotherapy including screening for polymorphisms to choose agents with the greatest potential for efficacy and least risk of toxicity. Pharmacogenetics also informs dose adaptations for specific drugs in patients with aberrant metabolism. Cardiovascular diseases (CVD) are considered one of the leading causes of death worldwide. To prevent cardiovascular complications and further loss of life oral anticoagulants (e.g., warfarin) are frequently prescribed to patients. Nevertheless, warfarin therapeutic agent presents narrow therapeutic windows with well-documented health risks. Some of these dose-responses are a result of specific single-nucleotide polymorphism (SNP) genetic variations present in a patient´s DNA. Among them, determined SNP in the cytochrome P4502C9 (CYP2C9), namely the CYP2C9*2, gene has been identified as dose-response altering SNP. Therefore, the need for a rapid, selective, low-cost and in real time detection device is crucial before prescribing any anticoagulant. In this work an analytical approach based on electrochemical genosensor technique is under development to create a low-cost genotyping platform able to genotype SNPs related with the therapeutic response of warfarin. Analyzing public databases, two specific 71 bp DNA probes, one with adenine (TA) and other with guanine (TG) SNP genetic variation were selected and designed. The design of this electrochemical genosensor consists of ssDNA immobilization onto gold surfaces that act as the SNPs complementary probes. The hybridization reaction is performed in a sandwich format of the complementary ssDNA, using an enzymatic scheme to amplify the electrochemical signal. The electrochemical signal was performed by using chronoamperometric technique.pt_PT
dc.description.versioninfo:eu-repo/semantics/publishedVersionpt_PT
dc.identifier.citationSousa, A. J. S. F., Costa, I., Banegas, R. S., Morais, S. L., Magalhães, J., Rodrigues, V., Delerue-Matos, C., Ramos-Jesus, J., Ferreira-Fernandes, H., R. Pinto, G., Santos, M., & Barroso, M. F. (2022, maio). Development of electrochemical genosensors for the CYPC*2 gene polymorphism detection [Poster]. 15º Encontro de Investigação Jovem da U.Porto, Portopt_PT
dc.identifier.isbn978-989-746-329-7
dc.identifier.urihttp://hdl.handle.net/10400.22/23533
dc.language.isoengpt_PT
dc.publisherUniversidade do Portopt_PT
dc.relation.publisherversionhttps://www.up.pt/ijup/wp-content/uploads/sites/892/2023/05/Livro_de_Resumos_versao_final-rev2.pdfpt_PT
dc.titleDevelopment of electrochemical genosensors for the CYPC*2 gene polymorphism detectionpt_PT
dc.typeconference object
dspace.entity.typePublication
oaire.citation.conferencePlacePortopt_PT
oaire.citation.endPage507pt_PT
oaire.citation.startPage506pt_PT
oaire.citation.titleLivro de Resumos do 15.º Encontro de Investigação Jovem da U.Portopt_PT
person.familyNameSantos
person.givenNameMarlene
person.identifier1508370
person.identifier.ciencia-id8311-B967-31C4
person.identifier.orcid0000-0001-5020-5942
person.identifier.scopus-author-id57110502000
rcaap.rightsopenAccesspt_PT
rcaap.typeconferenceObjectpt_PT
relation.isAuthorOfPublication8ce9ee39-a4c6-46ae-99e2-49397b550f1b
relation.isAuthorOfPublication.latestForDiscovery8ce9ee39-a4c6-46ae-99e2-49397b550f1b

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