| Name: | Description: | Size: | Format: | |
|---|---|---|---|---|
| 95.84 KB | Adobe PDF |
Advisor(s)
Abstract(s)
Growing concern about the contamination of wastewaters by antibiotics demands
fast but sensitive analytical methodologies, for the screening of a large number of
samples. The purpose of this work was to develop a simple methodology, using
direct injection of the samples, by HPLC with diode array detection (DAD), for a multiresidue
analysis of five antibiotics of different classes. Wastewater from an urban
water treatment plant was selected as a model to study possible coelution of interfering
compounds. The linearity interval ranged from 40 to 400 µg/L for amoxicillin
(Amox), metronidazole (Metro), cefazolin (Cefa), and chloramphenicol (Chloram)
and from 20 to 200 µg/L for sulfamethoxazole (Sulfa), with LODs lower than 14 µg/L.
Repeatability, expressed by the CV of six repeated injections, ranged from 1 to 8%,
while the intermediate precision varied between 2 and 11%. The recovery ranged
from 90 to 109%. This method enables the fast screening of a large number of samples,
with an expanded uncertainty in the 1–22% range. The advantage of the proposed
method is to significantly reduce the number of samples to be analyzed by
more complex methods.
Description
Keywords
Antibiotics HPLC-DAD Screening Uncertainty Wastewater
Citation
Publisher
WILEY-VCH Verlag GmbH & Co.