Lopes, InêsCampos, CarlaSilva, Fernandoda Costa, Rui M. GilFerreira, Layana S.da Rocha, Cláudia Q.Brito, Haissa O.Capela, João PauloPinto, EugéniaMedeiros, RuiCerqueira, Fátima2026-05-292026-05-292025-12-16Lopes, I., Campos, C., Silva, F., da Costa, R. M. G., Ferreira, L. S., da Rocha, C. Q., Brito, H. O., Capela, J. P., Pinto, E., Medeiros, R., & Cerqueira, F. (2025). Antimicrobial and anti-inflammatory activities of Brachydins A-C and root extracts from Fridericia platyphylla. Revista Brasileira de Farmacognosia, 36(1), 9. https://doi.org/10.1007/s43450-025-00728-70102-695Xhttp://hdl.handle.net/10400.22/32457A hydroethanolic extract and its nonpolar dichloromethane fraction obtained from roots of Fridericia platyphylla (Cham.) L.G.Lohmann, Bignoniaceae, were evaluated against yeasts (Candida albicans, C. krusei, and Cryptococcus neoformans), and Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. Neither extract showed relevant activity against yeasts or Gram-negative bacteria. In contrast, the dichloromethane fraction exhibited stronger activity against methicillin-sensitive S. aureus (MSSA, ATCC 29213) with minimum inhibitory concentration of 26.7 µg/ml and showed marked antioxidant capacity. Three dimeric flavonoids—brachydins A, B, and C—isolated from dichloromethane fraction displayed potent antimicrobial activity against MSSA ATCC 29213 and V459 strains, with brachydin C being the most active (MIC 12.5 and 75 µM, respectively). Brachydin C also decreased the activity of S. aureus virulence-associated enzymes (DNase and catalase). Extracts and brachydins inhibited nitric oxide (NO) production by LPS-stimulated RAW264.7 macrophages. The in silico ADME profile was further investigated. In conclusion, the results highlight the potential of brachydin C as an anti-staphylococcal agent and the antibacterial, anti-inflammatory and antioxidant potential of F. platyphylla.engDimeric flavonoidsAntimicrobialMinimum inhibitory concentrationVirulence-associated enzymesresearch article10.1007/s43450-025-00728-71981-528X