Rebelo, AndreiaMourão, JoanaFreitas, AnaCoque, TeresaAlmeida, AgostinhoPeixe, LuísaAntunes, PatríciaNovais, Carla2024-12-182024-12-182017-12Rebelo, A., Mourão, J., Freitas, A., Coque, T., Almeida, A., Peixe, Luísa, Antunes, P., & Novais, C. (2017). Distribution of Arsenic Tolerance Genes (arsA) among Enterococcus spp. From different sources, continents and timeframes (<1906-2015). Book of abstracts - Congress of Microbiology and Biotechnology 2017 - MICROBIOTEC 17, 149.http://hdl.handle.net/10400.22/26905Successful bacteria accumulate different genetic features shaping their evolution and fitness to diverse environments/hosts. Arsenic-compounds are widespread in nature and possibly contribute to selection of particular strains. The aim of this study was to evaluate the dispersion of arsA genes among Enterococcus from different sources, continents and timeframes. The arsA genes (coding for arsenical-pump-driving-ATPases) were searched in GenBank Enterococcus genomes and used to construct a maximum-likelihood phylogenetic-tree. A PCR scheme+sequencing was developed to detect arsA alleles from all phylogenetic subgroups identified among 333 isolates (Portugal; human/animal/environment/food; 1996-2012)1. Na2HAsO4 susceptibility was evaluated by agar dilution (0,25 to 128mM; n=143 isolates).engEnterococcusArsenic-compoundsMetal-tolerancePhylogenyconference object