Magalhães, SofiaLuís, CarlaFraga, HugoDuarte, Abel J.2024-05-032024-05-032023Magalhães, S., Luís, C., Fraga, H., & Duarte, A. (2023, May 10-12). Characterization of carbon dots and its impact in a breast cancer cell line [Poster]. IJUP - Investigação Jovem da Universidade do Porto, 16ª ed., Porto, Portugalhttp://hdl.handle.net/10400.22/25461This work was supported by DGES (Direção Geral de Ensino Superior) by a scholarship granted to Sofia Magalhães (number 1581670).Carbon dots (CD) have emerged as a novel promising alternative to metal-based nanoparticles. These fluorescence carbon materials have been the subject of research since 2004 and exhibit excellent chemical and physical properties such as hydrophilicity, good biocompatibility and reduced toxicity, which allows a good interaction with biological systems. Taking this in consideration it is not surprising that CD have been introduced for the study/therapeutics of breast cancer , especially, in drug delivery system, bioimaging, biosensor, microbial therapy, photodynamic and photothermal therapy. Breast cancer is the most prevalent cancer with a high morbidity rate in the female population worldwide. In this study, we considered the use of fructose derived CD for breast cancer therapy. CD were produced from D-fructose via microwave irradiation by the bottom-up method. Following purification, particles were characterized using TEM, FTIR, DLS and fluorescence microscopy. As expected, fructose derived CD consist of carbon, oxygen and hydrogen. As characteristic of CD, they are fluorescent with an absorption maximum at 360 nm that when irradiated at 360 nm have an emission maximum at 520 and a broad emission band (maximum 520 nm). In what concern cell-based assays, our results using breast cancer cell line BT474 and MCF-10A breast epithelial cells show CD are able to penetrate the cell membrane and display cytotoxicity towards tumor cell lines. In still preliminary results, it appears that they have reduced toxicity versus non tumor cell line, suggesting that they can be used as anti-tumoral agents for therapeutic purposes.engCarbon dotsNanoparticlesBreast cancerCell cultureconference object