Teixeira, DulcePrudêncio, CristinaFernandes, RúbenVieira, Mónica2022-03-092022-03-092015-11Teixeira, D., Prudêncio, C., Fernandes, R., & Vieira, M. (2015). Development of a new method for the detection and quantification of 3-nitrotyrosine. In R. A. Silva, P. Baylina, F. Q. Vieira, C. Barrias, M. Rubim, T. Coelho, C. Prudêncio, & Â. Jesus (Eds.), II Congresso Internacional da Saúde Gaia Porto: Livro de Resumos (1st ed., Vol. 1, pp. 135). Escola Superior de Tecnologia da Saúde do Instituto Politécnico do Porto.978-989-20-6340-9http://hdl.handle.net/10400.22/20188The nitration of Tyrosine residues in proteins is associated with nitrosative stress, resulting in the formation of 3-nitrotyrosine (3-NT). Different methods have been described for the quantification of this molecule. Regarding chromatographic methods, they seem to be very accurate, showing very good sensibility and specificity. Gas chromatography-based methods exhibit the highest sensibility, however a derivatization step prior analysis is required, which ends up being time-consuming for the analyst. Conversely, High Performance Liquid Chromatography (HPLC) may not require such derivatization.engconference object