Carvalho, SusanaCardoso, AndreiaFerreira, DéboraSilva, Diana Dias daMoreira, FernandoMoreira, FernandoDias da Silva, Diana Cristina2025-10-022025-10-022025-06-12Carvalho, S., Cardoso, A., Ferreira, D., Dias da Silva, D., & Moreira, F. (2025). Influence of puncture devices on the accuracy of cyclophosphamide dosing for chemotherapy administration. Pharmaceuticals, 18(6), 879. https://doi.org/10.3390/ph18060879http://hdl.handle.net/10400.22/30533Cyclophosphamide is one of the most commonly used cytotoxic drugs in chemotherapy protocols. Its preparation in the hospital setting involves handling concentrated solutions, which pose occupational exposure risks and potential variations in the final dose administered. The aim of this study was to evaluate the effect of aspiration devices on the concentration of cyclophosphamide in reconstituted solutions. An analytical method was validated using high-performance liquid chromatography coupled to a diode-array detector (HPLC-DAD) for quality control. Cyclophosphamide solutions were prepared and aspirated using either a conventional needle or spike device with or without a filtration system. The validated method demonstrated linearity (R2 = 0.9999), high precision (0.22–4.59%) and accuracy (88.9–99.4%), with a limit of quantification of 4.03 µg/mL. Significant differences (p < 0.001) were observed between samples aspirated with a needle and those aspirated with a spike fitted with a 5 µm filter, with the latter showing lower cyclophosphamide concentrations, suggesting partial retention of the drug. No significant differences were found between the needle and filterless spike preparations. These results suggest that the choice of aspiration device influences the final drug concentration, potentially affecting therapeutic efficacy. Standardisation of preparation techniques and an awareness of device limitations are essential to ensure accurate chemotherapy dosing and patient safety.engHigh-performance liquid chromatography with diode-array detection (HPLC-DAD)CyclophosphamideAnticancer drugsChemotherapy preparationAspiration devicesQuality controlDose accuracyresearch articlehttps://www.mdpi.com/1424-8247/18/6/8791424-8247