Costa-Rodrigues, JoãoCosta, M. M.Costa, S.Garcia, M.Fernandes, M. H.Vasconcelos, V.Barros, PiedadeMartins, Rosário2021-09-282021-09-282012-05Costa-Rodrigues, J., Costa, M., Costa, S., Garcia, M., Fernandes, M. H., Vasconcelos, V., Barros, P., & Martins, R. (2012). Cytotoxicity of marine cyanobacteria extracts on osteosarcoma cells. Bone, 50, S181-S182. https://doi.org/https://doi.org/10.1016/j.bone.2012.02.5748756-3282http://hdl.handle.net/10400.22/18608Marine cyanobacteria have been identified as a rich source of secondary metabolites with potential pharmacological applications. Anti-inflammatory, antibacterial and anticancer activities are some examples of properties described for cyanobacteria compounds, being the cytoxicity against cancer cell lines one of the most documented. The Laboratory of Ecotoxicology Genomics and Evolution (LEGE) — CIIMAR, Porto, Portugal, possesses a large collection of cyanobacteria strains isolated from the Portuguese coast. In order to investigate the interest of genera such as Cyanobium, Synechocystis, Synechococcus, Leptolyngbya and Pseudoanabaena which are genera that have been largely overlooked in terms of bioactivity, we have been screening their ability to induce cytotoxicity on human cancer cell lines. Assays have been conducted with a crude extract obtained by a dichloromethane and methanol extraction of freeze dried biomass and three fractions obtained using Si column chromatography with a gradient from 100% hexane, to 100% ethyl acetate to 100% methanol. The cytotoxicity of cyanobacteria crude extract and fractions has been evaluated by the MTT assay at 24, 48 and 72 h. Here we present the results concerning the cytotoxicity of 24 cyanobacteria strains on the osteosarcoma cell line MG63. The results show both inhibitory and stimulatory effects on cell growth within the same cyanobacteria strain. However, five cyanobacteria strains were found to induce a decrease in cell viability that reached the 80% within the ethyl acetate fraction, which makes this fraction interesting for the isolation and characterization of new bioactive compounds.engCyanobacteriaCytoxicityconference object10.1016/j.bone.2012.02.574