Almeida, JoanaCosta, J.Coelho, PedroCea, V.Galesio, M.Noronha, J. P.Diniz, M. S.Prudêncio, CristinaSoares, R.Sala, C.Fernandes, Rúben2019-07-012020-05-022018Almeida, J., Costa, J., Coelho, P., Cea, V., Galesio, M., Noronha, J. P., Diniz, M. S., Prudêncio, C., Soares, R., Sala, C., & Fernandes, R. (2019). Adipocyte proteome and secretome influence inflammatory and hormone pathways in glioma. Metabolic Brain Disease, 34(1), 141–152. https://doi.org/10.1007/s11011-018-0327-yhttp://hdl.handle.net/10400.22/14213Gliomas represent the most common primary malignant brain tumors in adults, with an extremely poor prognosis. Among several risk factors, lifestyle was also recently identified as a major risk factor for the development of primary glioma. In the present study, we explore the relationship between obesity and glioma in a cellular model. Thus, we have study the influence of adipocytes secretome on glioma cell line GL261. Using the 3T3-L1 adipocyte cell line, and its conditioned medium (adipokines-enriched medium), we showed that adipocyte-released factors relate with glioma angiogenic, growth, hormones and metabolic behavior by MALDI-TOF-MS and proteomic array analysis. In a first view, STI1, hnRNPs and PGK1 are under expressed on CGl. Similarly, both carbonic anhydrase and aldose reductase are even suppressed in glioma cells that grown under adipokines-enriched environment. Contrariwise, RFC1, KIF5C, ANXA2, N-RAP and RACK1 are overexpressed in GL261 cell the in the presence of the adipokines-enriched medium. We further identified the factors that are released by adipocyte cells, and revealed that several pro-inflammatory and angiogenic factors, such as IL-6, IL-11, LIF, PAI-1, TNF-α, endocan, HGF, VEGF IGF-I, were secreted to the medium into a high extent, whereas TIMP-1 and SerpinE1 were under expressed on CGl. This study discloses an interesting in vitro model for the study of glioma biology under a "obesity" environment, that can be explored for the understanding of cancer cells biology, for the search of biomarkers, prognostic markers and therapeutic approaches.eng3T3-L1 CellsAdipocytesAnimalsBrain NeoplasmsCell DifferentiationCell Line, TumorGliomaInflammationProteomeProteomicsjournal article10.1007/s11011-018-0327-y