Teixeira, FilipaSilva, Ana MargaridaSut, StefaniaDall’Acqua, StefanoRamos, Oscar L.Ribeiro, Alessandra B.Ferraz, RicardoDelerue-Matos, CristinaRodrigues, Francisca2024-05-292024Teixeira, F., Silva, A. M., Sut, S., Dall’Acqua, S., Ramos, O. L., Ribeiro, A. B., Ferraz, R., Delerue-Matos, C., & Rodrigues, F. (2024). Ultrasound-assisted extraction of bioactive compounds from goji berries: Optimization, bioactivity, and intestinal permeability assessment. Food Research International, 188, 114502. https://doi.org/10.1016/j.foodres.2024.1145020963-99691873-7145http://hdl.handle.net/10400.22/25587valuable component of health and nutraceutical practices. Nevertheless, a deep insight on the intestinal permeation of the pro-healthy bioactive compounds is urgently needed to predict the real effects on human body. This study attempted, for the first time, to optimize the Ultrasound-Assisted Extraction (UAE) of goji berries using a Response Surface Methodology approach and establish the intestinal permeation of the principal pro-healthy compounds. The optimal extraction conditions were a solid:liquid ratio of 8.75 % for 56.21 min, using an intensity of 59.05 W/m2 . The optimal extract displayed a remarkable antioxidant capacity, with LC/DAD-ESI-MS analysis unveiled a diverse phytochemical profile, encompassing different compounds (e.g. glulycibarbarspermidine F, 2-glu-kukoamine, rutin, 3,5-dicaffeoylquinic acid). The intestinal co-culture model demonstrated that glu-lycibarbarspermidine F (isomer 2) (73.70 %), 3,5-dicaffeoylquinic acid (52.66 %), and isorhamnetin-3-O-rutinoside (49.31 %) traversed the intestinal cell layer, exerting beneficial health-promoting effects.engLycium barbarumResponse surface methodologyUltrasound-assisted extractionPhenolic compoundsIntestinal permeationjournal article10.1016/j.foodres.2024.114502