Neves, CátiaMorais, StephanieVale, TiagoSoares, CristinaGrosso, ClaraDomingues, Valentina F.Vieira, MónicaDelerue-Matos, CristinaVieira, Mónica2026-01-072026-01-072023-10-14Neves, C., Morais, S., Vale, T., Soares, C., Grosso, C., Domingues, V. F., Vieira, M., & Delerue-Matos, C. (2023). Exploring the bioactive potential of Gracilaria gracilis: An extraction optimization study using response surface methodology. The 4th International Electronic Conference on Foods - Biology and Life Sciences Forum, 26, 13. https://doi.org/10.3390/Foods2023-15026http://hdl.handle.net/10400.22/31412The extraction of bioactive compounds from the seaweed Gracilaria gracilis was optimized for food use using a Response Surface Methodology. Two designs, Central Composite Face-centered (CCD) and Box-Behnken (BBD) assessed the effects of extraction time, temperature, and seaweed-to-solvent ratio using water as the solvent. The extraction yield was assessed by Total Phenolic Content (TPC). BBD’s best model was a Reduced Quadratic (R2 = 0.9356), predicting 3.336 mg GAE/L at 74 °C in 1.4 h with a 1:75 ratio. CCD’s top was Reduced Cubic (R2 = 0.9091), forecasting 4.278 mg GAE/L at 46 °C in 1.1 h, the same ratio. The actual obtained TPC values were 4.35 mg GAE/L for BBD and 4.25 mg GAE/L for CCD.engAlgaeExtraction optimizationTotal phenolic compounds (TPC)conference paper10.3390/Foods2023-15026