Bessa, Lucinda JEaton, PeterDematei, AndersonPlácido, AlexandraVale, NunoGomes, PaulaDelerue-Matos, CristinaLeite, José Roberto SáGameiro, Paula2019-10-292019-10-2920181746-0913http://hdl.handle.net/10400.22/14762Aim:To test ocellatin peptides (ocellatins-PT2-PT6) for antibacterial and antibiofilm activities and synergy with antibiotics against Pseudomonas aeruginosa. Materials & methods: Normal- and checkerboard-broth microdilution methods were used. Biofilm studies included microtiter plate-based assays and microscopic analysis by confocal laser scanning microscopy and atomic force microscopy. Results: Ocellatins were more active against multidrug-resistant isolates of P. aeruginosa than against susceptible strains. Ocellatin-PT3 showed synergy with ciprofloxacin and ceftazidime against multidrug-resistant isolates and was capable of preventing the proliferation of 48-h mature biofilms at concentrations ranging from 4 to 8× the MIC. Treated biofilms had low viability and were slightly more disaggregated. Conclusion: Ocellatin-PT3 may be promising as a template for the development of novel antimicrobial peptides against P. aeruginosa.engAMPsAntibiofilm ActivityAntimicrobial peptidesMultidrug-resistant Pseudomonas aeruginosaOcellatin peptidesOcellatin-PT3Synergyjournal article10.2217/fmb-2017-0175