Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/9228
Título: Comprehensive Analysis of Secreted Protein, Acidic and Rich in Cysteine in Prostate Carcinogenesis: Development of a 3D Nanostructured Bone-Like Model
Autor: Ribeiro, Nilza
Costa-Pinheiro, Pedro
Henrique, Rui
Gomez-Lazaro, Maria
Pereira, Marisa P.
Mansur, Alexandra A. P.
Mansur, Herman S.
Jerónimo, Carmen
Freitas, Susana Maria Ribeiro e Sousa Mendes de
Monteiro, Fernando J.
Palavras-chave: 3D bone-biomimetic biomaterial
Bone metastasis
Gleason score
Prostate cancer
Quantum dots
Data: 2016
Editora: American Scientific Publishers
Relatório da Série N.º: Journal of Biomedical Nanotechnology;Vol. 12, nº 8
Resumo: Most aggressive prostate cancer (PCa) types tend to metastasize frequently to bone and SPARC, a matricellular protein, might participate in such biological processes. The objective of this study was to evaluate the effect of SPARC in prostate carcinogenesis and bone metastization. This was explored assessing the morphology, metabolic activity and SPARC expression of different PCa cell lines resembling different stages of carcinogenesis, using a 3D bone-biomimetic model (collagen nanofibers/nanohydroxyapatite) grafted with SPARC. Our findings highlight distinct cellular behavior depending on cell type and presence of exogenous SPARC. In fact, SPARC addition contributed to the survival and significant growth of a non-bone metastatic PCa cell line (LNCaP) on bone-like biomaterial. Moreover, SPARC expression levels were evaluated in a series of prostatic tissues, comparing normal prostate, pre-neoplastic prostate intraepithelial neoplasias and overtly malignant tumors, and also metastasis to its correspondent primary prostate tumors, ascertaining potential association between SPARC and clinicopathological data. Remarkably, SPARC was overexpressed in patients with higher Gleason Score, indicating tumors with poor prognosis, as well as in metastasis, particularly from bone sites, compared with their respective primary tumors. The results suggest a potential role of SPARC as a clinical target on PCa, due to its association with bone metastization.
URI: http://hdl.handle.net/10400.22/9228
DOI: https://doi.org/10.1166/jbn.2016.2276
ISSN: 1550-7033
Versão do Editor: http://www.ingentaconnect.com/content/asp/jbn/2016/00000012/00000008/art00007
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