Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/7255
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dc.contributor.authorAlves, Rita C.-
dc.contributor.authorPimentel, Filipa B.-
dc.contributor.authorNouws, Henri P.A.-
dc.contributor.authorMarques, Raquel C.B.-
dc.contributor.authorGonzález-García, María Begoña-
dc.contributor.authorOliveira, M. Beatriz P.P.-
dc.contributor.authorDelerue-Matos, Cristina-
dc.date.accessioned2015-12-23T16:06:55Z-
dc.date.available2015-12-23T16:06:55Z-
dc.date.issued2015-02-
dc.identifier.urihttp://hdl.handle.net/10400.22/7255-
dc.description.abstractA gold nanoparticle-coated screen-printed carbon electrode was used as the transducer in the development of an electrochemical immunosensor for Ara h 1 (a major peanut allergen) detection in food samples. Gold nanoparticles (average diameter=32 nm) were electrochemically generated on the surface of screen-printed carbon electrodes. Two monoclonal antibodies were used in a sandwich-type immunoassay and the antibody–antigen interaction was electrochemically detected through stripping analysis of enzymatically (using alkaline phosphatase) deposited silver. The total time of the optimized immunoassay was 3 h 50 min. The developed immunosensor allowed the quantification of Ara h 1 between 12.6 and 2000 ng/ml, with a limit of detection of 3.8 ng/ml, and provided precise (RSD <8.7%) and accurate (recovery >96.6%) results. The immunosensor was successfully applied to the analysis of complex food matrices (cookies and chocolate), being able to detect Ara h 1 in samples containing 0.1% of peanut.pt_PT
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.relation.ispartofseriesBiosensors and Bioelectronics;Vol. 64-
dc.rightsopenAccesspt_PT
dc.subjectElectrochemical immunosensorpt_PT
dc.subjectBiosensorpt_PT
dc.subjectScreen-printed carbon electrodept_PT
dc.subjectAllergenpt_PT
dc.subjectPeanutpt_PT
dc.subjectAra h 1pt_PT
dc.titleDetection of Arah1 (a major peanut allergen) in food using an electrochemical gold nanoparticle-coated screen-printed immunosensorpt_PT
dc.typearticlept_PT
degois.publication.firstPage19pt_PT
degois.publication.lastPage24pt_PT
degois.publication.titleBiosensors and Bioelectronicspt_PT
degois.publication.volume64pt_PT
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0956566314006149pt_PT
dc.identifier.doi10.1016/j.bios.2014.08.026pt_PT
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