Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/7255
Título: Detection of Arah1 (a major peanut allergen) in food using an electrochemical gold nanoparticle-coated screen-printed immunosensor
Autor: Alves, Rita C.
Pimentel, Filipa B.
Nouws, Henri P.A.
Marques, Raquel C.B.
González-García, María Begoña
Oliveira, M. Beatriz P.P.
Delerue-Matos, Cristina
Palavras-chave: Electrochemical immunosensor
Biosensor
Screen-printed carbon electrode
Allergen
Peanut
Ara h 1
Data: Fev-2015
Editora: Elsevier
Relatório da Série N.º: Biosensors and Bioelectronics;Vol. 64
Resumo: A gold nanoparticle-coated screen-printed carbon electrode was used as the transducer in the development of an electrochemical immunosensor for Ara h 1 (a major peanut allergen) detection in food samples. Gold nanoparticles (average diameter=32 nm) were electrochemically generated on the surface of screen-printed carbon electrodes. Two monoclonal antibodies were used in a sandwich-type immunoassay and the antibody–antigen interaction was electrochemically detected through stripping analysis of enzymatically (using alkaline phosphatase) deposited silver. The total time of the optimized immunoassay was 3 h 50 min. The developed immunosensor allowed the quantification of Ara h 1 between 12.6 and 2000 ng/ml, with a limit of detection of 3.8 ng/ml, and provided precise (RSD <8.7%) and accurate (recovery >96.6%) results. The immunosensor was successfully applied to the analysis of complex food matrices (cookies and chocolate), being able to detect Ara h 1 in samples containing 0.1% of peanut.
URI: http://hdl.handle.net/10400.22/7255
DOI: 10.1016/j.bios.2014.08.026
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0956566314006149
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