Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/7253
Título: 3D-nanostructured Au electrodes for the event-specific detection of MON810 transgenic maize
Autor: Barroso, M. Fátima
Freitas, Maria
Oliveira, M. Beatriz P.P.
de-los-Santos-Álvarez, Noemí
Lobo-Castañón, María Jesús
Delerue-Matos, Cristina
Palavras-chave: GMO
Genosensor
Nanostructured 3D Au electrodes
Screen-printed electrodes
MON810 maize
Data: Mar-2015
Editora: Elsevier
Relatório da Série N.º: Talanta;Vol. 134
Resumo: In the present work, the development of a genosensor for the event-specific detection of MON810 transgenic maize is proposed. Taking advantage of nanostructuration, a cost-effective three dimensional electrode was fabricated and a ternary monolayer containing a dithiol, a monothiol and the thiolated capture probe was optimized to minimize the unspecific signals. A sandwich format assay was selected as a way of precluding inefficient hybridization associated with stable secondary target structures. A comparison between the analytical performance of the Au nanostructured electrodes and commercially available screen-printed electrodes highlighted the superior performance of the nanostructured ones. Finally, the genosensor was effectively applied to detect the transgenic sequence in real samples, showing its potential for future quantitative analysis.
URI: http://hdl.handle.net/10400.22/7253
DOI: 10.1016/j.talanta.2014.10.017
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0039914014008455
Aparece nas colecções:ISEP – GRAQ – Artigos

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