Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/7236
Título: New approach for vitamin E extraction in rainbow trout flesh: Application in fish fed commercial and red seaweed-supplemented diets
Autor: Araújo, Mariana
Alves, Rita C.
Pimentel, Filipa B.
Costa, Anabela S.G.
Fernandes, Telmo J.R.
Valente, Luísa M.P.
Rema, Paulo
Oliveira, M. Beatriz P.P.
Palavras-chave: Vitamin E
Rainbow trout flesh
Gracilaria vermiculophylla
Extraction method
Data: Jun-2015
Editora: WILEY-VCH
Relatório da Série N.º: European Journal of Lipid Science and Technology;Vol. 117, Issue 9
Resumo: A vitamin E extraction method for rainbow trout flesh was optimized, validated, and applied in fish fed commercial and Gracilaria vermiculophylla-supplemented diets. Five extraction methods were compared. Vitamers were analyzed by HPLC/DAD/fluorescence. A solid-liquid extraction with n-hexane, which showed the best performance, was optimized and validated. Among the eight vitamers, only α- and γ-tocopherol were detected in muscle samples. The final method showed good linearity (>0.999), intra- (<3.1%) and inter-day precision (<2.6%), and recoveries (>96%). Detection and quantification limits were 39.9 and 121.0 ng/g of muscle, for α-tocopherol, and 111.4 ng/g and 337.6 ng/g, for γ-tocopherol, respectively. Compared to the control group, the dietary inclusion of 5% G. vermiculophylla resulted in a slight reduction of lipids in muscle and, consequently, of α- and γ-tocopherol. Nevertheless, vitamin E profile in lipids was maintained. In general, the results may be explained by the lower vitamin E level in seaweed-containing diet. Practical Applications: Based on the validation results and the low solvent consumption, the developed method can be used to analyze vitamin E in rainbow trout. The results of this work are also a valuable information source for fish feed industries and aquaculture producers, which can focus on improving seaweed inclusion in feeds as a source of vitamin E in fish muscle and, therefore, take full advantage of all bioactive components with an important role in fish health and flesh quality.
URI: http://hdl.handle.net/10400.22/7236
DOI: 10.1002/ejlt.201400656
Versão do Editor: http://onlinelibrary.wiley.com/doi/10.1002/ejlt.201400656/full
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