QuEChERS: a sample preparation for extraction of carbaryl from rat feces

Paíga, Paula; Domingues, Valentina F.; Wanderley, Kaline Amaral; Júnior, Severino Alves; Delerue-Matos, Cristina

http://hdl.handle.net/10400.22/7225

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Authors

Paíga, Paula

Domingues, Valentina F.

Wanderley, Kaline Amaral

Júnior, Severino Alves

Delerue-Matos, Cristina

Abstract(s)

Carbamate compounds are an important group of cholinesterase inhibitors. There is a need for creating awareness regarding the risks of the inadequate carbamate use in the residential areas due to potential adverse human effects. Carbaryl is a commonly used pesticide worldwide. A simple, fast, and high throughput method was developed employing liquid chromatography with fluorescence detector to determine carbaryl residues in rat feces. The extraction was performed by using a rapid, easy, cheap, effective, reliable, and safe (QuEChERS) method, using acetonitrile as the extracting solvent. The parameters for the performance of the extraction method were optimized, such as ratio of mass of sample per volume of extraction solvent, QuEChERS content, and cleanup columns. Linear response was obtained for all calibration curves (solven and matrix-matched) over the established concentration range (5 500 mg/L) with a correlation coefficients higher than 0.999. The achieved recovery was 97.9% with relative standard deviation values of 1.1% (n D 4) at 167 mg/kg fortified concentration level and the limits of detection and quantification were 27.7 and 92.3 mg/kg respectively.