Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/7224
Título: Detection of the peanut allergen Ara h 6 in foodstuffs using a voltammetric biosensing approach
Autor: Alves, Rita C.
Pimentel, Filipa B.
Nouws, Henri P. A.
Correr, Wagner
González-García, María Begoña
Oliveira, M. Beatriz P. P.
Delerue-Matos, Cristina
Palavras-chave: Electrochemical immunosensor
Ara h 6
Voltammetry
Screen-printed carbon electrodes
Allergen
Peanut
Data: Jul-2015
Editora: Springer
Relatório da Série N.º: Analytical and Bioanalytical Chemistry;Vol. 407, Issue 23
Resumo: A voltammetric biosensor for Ara h 6 (a peanut allergen) detection in food samples was developed. Gold nanoparticle-modified screen-printed carbon electrodes were used to develop a sandwich-type immunoassay using two-monoclonal antibodies. The antibody-antigen interaction was detected through the electrochemical detection of enzymatically deposited silver. The immunosensor presented a linear range between 1 and 100 ng/ml, as well as high precision (inter-day RSD ≤9.8 %) and accuracy (recoveries ≥96.7 %). The detection and quantification limits were 0.27 and 0.88 ng/ml, respectively. It was possible to detect small levels of Ara h 6 in complex food matrices.
Peer review: yes
URI: http://hdl.handle.net/10400.22/7224
DOI: 10.1007/s00216-015-8879-8
ISSN: 1618-2650
Versão do Editor: http://link.springer.com/article/10.1007/s00216-015-8879-8
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