Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/7221
Título: Molecularly imprinted electrochemical sensor for ochratoxin A detection in food samples
Autor: Pacheco, João G.
Castro, Mafalda
Machado, Susana
Barroso, M. Fátima
Nouw, Henri P.A.
Delerue-Matos, Cristina
Palavras-chave: Ochratoxin A
Electrochemical sensor
Molecularly imprinted polymer
Multiwalled carbon nanotubes
Data: Ago-2015
Editora: Elsevier
Relatório da Série N.º: Sensors and Actuators B: Chemical;Vol. 215
Resumo: A novel electrochemical sensor for ochratoxin A (OTA) detection was fabricated through the modification of a glassy carbon electrode (GCE) with multiwalled carbon nanotubes (MWCNTs) and a molecularly imprinted polymer (MIP). The MWCNTs dramatically promoted the sensitivity of the developed sensor, while polypyrrole (PPy) imprinted with OTA served as the selective recognition element. The imprinted PPy film was prepared by electropolymerization of pyrrole in the presence of OTA as a template molecule via cyclic voltammetry (CV). The electrochemical oxidation of OTA at the developed sensor was investigated by CV and differential pulse voltammetry (DPV). The developed MIP/MWCNT/GCE sensor showed a linear relationship, when using DPV, between peak current intensity and OTA concentration in the range between 0.050 and 1.0 μM, with limits of detection (LOD) and quantification of 0.0041 μM (1.7 μg/L) and 0.014 μM (5.7 μg/L) respectively. With the developed sensor precise results were obtained; relative standard deviations of 4.2% and 7.5% in the evaluation of the repeatability and reproducibility, respectively. The MIP/MWCNT/GCE sensor is simple to fabricate and easy to use and was successfully applied to the determination of OTA in spiked beer and wine samples, with recoveries between 84 and 104%, without the need of a sample pre-treatment step.
Peer review: yes
URI: http://hdl.handle.net/10400.22/7221
DOI: 10.1016/j.snb.2015.03.046
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0925400515003706
Aparece nas colecções:ISEP – GRAQ – Artigos

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
ART_PachecoJoao_2015_GRAQ.pdf730,9 kBAdobe PDFVer/Abrir    Acesso Restrito. Solicitar cópia ao autor!


FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpace
Formato BibTex MendeleyEndnote Degois 

Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.