Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/7203
Título: A potentiometric magnetic immunoassay for rapid detection of Salmonella typhimurium
Autor: Silva, Nádia F. D.
Magalhães, Júlia M. C. S.
Oliva-Teles, Teresa
Delerue-Matos, Cristina
Data: Abr-2015
Editora: Royal Society of Chemistry
Relatório da Série N.º: Analytical Methods;Vol. 7, Issue 9
Resumo: Potentiometric detection with homemade polymeric membrane microelectrodes was coupled to a magnetic sandwich immunoassay for Salmonella typhimurium determination. Cadmium and sodium ion selective electrodes were used respectively as indicator and pseudo-reference electrodes and were prepared in pipette tips to allow potentiometric measurements in microliter sample volumes. In the proposed method, the concentration of S. typhimurium was proportional to the amount of cadmium released upon dissolution of a CdS nanoparticle labeled to the secondary detection antibody. The limit of detection was 2 cells per 100 μL. The immunomagnetic assay with potentiometric detection is suitable for sensitive and rapid (average total time per assay of 75 minutes) detection of S. typhimurium in milk samples. The proposed method is easy to perform, safe, sensitive, and low cost and has potential for in situ analysis.
Peer review: yes
URI: http://hdl.handle.net/10400.22/7203
DOI: 10.1039/C5AY00053J
Versão do Editor: http://pubs.rsc.org/en/content/articlelanding/2015/ay/c5ay00053j/unauth#!divAbstract
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