Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/6752
Título: Artificial antibodies for troponin T by its imprinting on the surface of multiwalled carbon nanotubes: Its use as sensory surfaces
Autor: Moreira, Felismina T. C.
Dutra, Rosa A.F.
Noronha, João P. C.
Cunha, Alexandre L.
Sales, M. Goreti F.
Palavras-chave: Artificial antibody
Troponin T
Molecularly imprinted biomaterials
Potentiometric sensor
Cardiac biomarker
Data: 2011
Editora: Elsevier
Resumo: A novel artificial antibody for troponin T (TnT) was synthesized by molecular imprint (MI) on the surface of multiwalled carbon nanotubes (MWCNT). This was done by attaching TnT to the MWCNT surface, and filling the vacant spaces by polymerizing under mild conditions acrylamide (monomer) in N,N′-methylenebisacrylamide (cross-linker) and ammonium persulphate (initiator). After removing the template, the obtained biomaterial was able to rebind TnT and discriminate it among other interfering species. Stereochemical recognition of TnT was confirmed by the non-rebinding ability displayed by non-imprinted (NI) materials, obtained by imprinting without a template. SEM and FTIR analysis confirmed the surface modification of the MWCNT. The ability of this biomaterial to rebind TnT was confirmed by including it as electroactive compound in a PVC/plasticizer mixture coating a wire of silver, gold or titanium. Anionic slopes of 50 mV decade−1 were obtained for the gold wire coated with MI-based membranes dipped in HEPES buffer of pH 7. The limit of detection was 0.16 μg mL−1. Neither the NI-MWCNT nor the MWCNT showed the ability to recognize the template. Good selectivity was observed against creatinine, sucrose, fructose, myoglobin, sodium glutamate, thiamine and urea. The sensor was tested successfully on serum samples. It is expected that this work opens new horizons on the design of new artificial antibodies for complex protein structures.
URI: http://hdl.handle.net/10400.22/6752
DOI: 10.1016/j.bios.2011.07.026
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0956566311004544
Aparece nas colecções:ISEP – BioMark – Artigos

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
ART_GoretiSales_2011.pdf1,19 MBAdobe PDFVer/Abrir


FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpace
Formato BibTex MendeleyEndnote Degois 

Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.