Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/6731
Título: Novel biosensing device for point-of-care applications with plastic antibodies grown on Au-Screen Printed Electrodes
Autor: Moreira, Felismina T. C.
Dutra, Rosa A.F.
Noronha, João P. C.
Fernandes, João S.
Sales, M. Goreti F.
Palavras-chave: Surface molecular imprint
Self-assembled monolayer
Screen-printed electrodes
Cardiac biomarker
Myoglobin
Biosensor
Data: 2013
Editora: Elsevier
Resumo: A gold screen printed electrode (Au-SPE) was modified by merging Molecular Imprinting and Self-Assembly Monolayer techniques for fast screening cardiac biomarkers in point-of-care (POC). For this purpose, Myoglobin (Myo) was selected as target analyte and its plastic antibody imprinted over a glutaraldehyde (Glu)/cysteamine (Cys) layer on the gold-surface. The imprinting effect was produced by growing a reticulated polymer of acrylamide (AAM) and N,N′-methylenebisacrylamide (NNMBA) around the Myo template, covalently attached to the biosensing surface. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) studies were carried out in all chemical modification steps to confirm the surface changes in the Au-SPE. The analytical features of the resulting biosensor were studied by different electrochemical techniques, including EIS, square wave voltammetry (SWV) and potentiometry. The limits of detection ranged from 0.13 to 8 μg/mL. Only potentiometry assays showed limits of detection including the cut-off Myo levels. Quantitative information was also produced for Myo concentrations ≥0.2 μg/mL. The linear response of the biosensing device showed an anionic slope of ~70 mV per decade molar concentration up to 0.3 μg/mL. The interference of coexisting species was tested and good selectivity was observed. The biosensor was successfully applied to biological fluids.
URI: http://hdl.handle.net/10400.22/6731
DOI: 10.1016/j.snb.2013.03.099
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0925400513003882
Aparece nas colecções:ISEP – BioMark – Artigos

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