Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/6729
Registo completo
Campo DCValorIdioma
dc.contributor.authorMoreira, Felismina T. C.-
dc.contributor.authorDutra, Rosa A.F.-
dc.contributor.authorNoronha, João P. C.-
dc.contributor.authorSales, M. Goreti F.-
dc.date.accessioned2015-10-16T12:11:55Z-
dc.date.available2015-10-16T12:11:55Z-
dc.date.issued2013-
dc.identifier.urihttp://hdl.handle.net/10400.22/6729-
dc.description.abstractA novel reusable molecularly imprinted polymer (MIP) assembled on a polymeric layer of carboxylated poly(vinyl chloride) (PVCsingle bondCOOH) for myoglobin (Myo) detection was developed. This polymer was casted on the gold working area of a screen printed electrode (Au-SPE), creating a novel disposable device relying on plastic antibodies. Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and Fourier transform infrared spectroscopy (FTIR) studies confirmed the surface modification. The MIP/Au-SPE devices displayed a linear behaviour in EIS from 0.852 to 4.26 μg mL−1, of positive slope 6.50 ± 1.48 (kΩ mL μg−1). The limit of detection was 2.25 μg mL−1. Square wave voltammetric (SWV) assays were made in parallel and showed linear responses between 1.1 and 2.98 μg mL−1. A current decrease was observed against Myo concentration, producing average slopes of −0.28 ± 0.038 μA mL μg−1. MIP/Au-SPE also showed good results in terms of selectivity. The error% found for each interfering species were 7% for troponin T (TnT), 11% for bovine serum albumin (BSA) and 2% for creatine kinase MB (CKMB), respectively. Overall, the technical modification over the Au-SPE was found a suitable approach for screening Myo in biological fluids.pt_PT
dc.language.isoengpt_PT
dc.publisherElsevierpt_PT
dc.rightsopenAccesspt_PT
dc.subjectBiosensorpt_PT
dc.subjectSurface molecular imprintpt_PT
dc.subjectScreen printed electrodespt_PT
dc.subjectSensorpt_PT
dc.subjectMyoglobinpt_PT
dc.titleElectrochemical biosensor based on biomimetic material for myoglobin detectionpt_PT
dc.typearticlept_PT
degois.publication.firstPage481pt_PT
degois.publication.lastPage487pt_PT
degois.publication.titleElectrochimica Actapt_PT
degois.publication.volume107pt_PT
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0013468613011675pt_PT
dc.identifier.doi10.1016/j.electacta.2013.06.061-
Aparece nas colecções:ISEP – BioMark – Artigos

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
ART_Felismina_2013.pdf1,21 MBAdobe PDFVer/Abrir


FacebookTwitterDeliciousLinkedInDiggGoogle BookmarksMySpace
Formato BibTex MendeleyEndnote 

Todos os registos no repositório estão protegidos por leis de copyright, com todos os direitos reservados.