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|Título:||Electrochemical biosensor based on biomimetic material for myoglobin detection|
|Autor:||Moreira, Felismina T. C.|
Dutra, Rosa A.F.
Noronha, João P. C.
Sales, M. Goreti F.
Surface molecular imprint
Screen printed electrodes
|Resumo:||A novel reusable molecularly imprinted polymer (MIP) assembled on a polymeric layer of carboxylated poly(vinyl chloride) (PVCsingle bondCOOH) for myoglobin (Myo) detection was developed. This polymer was casted on the gold working area of a screen printed electrode (Au-SPE), creating a novel disposable device relying on plastic antibodies. Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and Fourier transform infrared spectroscopy (FTIR) studies confirmed the surface modification. The MIP/Au-SPE devices displayed a linear behaviour in EIS from 0.852 to 4.26 μg mL−1, of positive slope 6.50 ± 1.48 (kΩ mL μg−1). The limit of detection was 2.25 μg mL−1. Square wave voltammetric (SWV) assays were made in parallel and showed linear responses between 1.1 and 2.98 μg mL−1. A current decrease was observed against Myo concentration, producing average slopes of −0.28 ± 0.038 μA mL μg−1. MIP/Au-SPE also showed good results in terms of selectivity. The error% found for each interfering species were 7% for troponin T (TnT), 11% for bovine serum albumin (BSA) and 2% for creatine kinase MB (CKMB), respectively. Overall, the technical modification over the Au-SPE was found a suitable approach for screening Myo in biological fluids.|
|Versão do Editor:||http://www.sciencedirect.com/science/article/pii/S0013468613011675|
|Aparece nas colecções:||ISEP – BioMark – Artigos|
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|ART_Felismina_2013.pdf||1,21 MB||Adobe PDF||Ver/Abrir|
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