Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/6722
Título: Multifunctional Biosensor Based on Localized Surface Plasmon Resonance for Monitoring Small Molecule–Protein Interaction
Autor: Guerreiro, J. Rafaela L.
Frederiksen, M.
Bochenkov, V. E.
Freitas, Victor De
Sales, M. Goreti F.
Palavras-chave: colloidal lithography
biosensing
localized surface plasmon resonance (LSPR)
small molecules
nanodisks
α-amylase
Data: 2014
Editora: ACS Publicatins
Resumo: We report an optical sensor based on localized surface plasmon resonance (LSPR) to study small-molecule protein interaction combining high sensitivity refractive index sensing for quantitative binding information and subsequent conformation-sensitive plasmon-activated circular dichroism spectroscopy. The interaction of α-amylase and a small-size molecule (PGG, pentagalloyl glucose) was log concentration-dependent from 0.5 to 154 μM. In situ tests were additionally successfully applied to the analysis of real wine samples. These studies demonstrate that LSPR sensors to monitor small molecule–protein interactions in real time and in situ, which is a great advance within technological platforms for drug discovery.
URI: http://hdl.handle.net/10400.22/6722
DOI: 10.1021/nn501962y
Versão do Editor: http://pubs.acs.org/doi/abs/10.1021/nn501962y
Aparece nas colecções:ISEP – BioMark – Artigos

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