Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/6277
Título: Culture-dependent characterization of cyanobacterial diversity in the intertidal zones of the Portuguese coast: A polyphasic study
Autor: Brito, Ângela
Ramos, Vitor
Seabra, Rui
Santos, Arlete
Santos, Catarina
Lopo, Miguel
Ferreira, Sérgio
Martins, António
Mota, Rita
Frazão, Bárbara
Martins, Rosário
Vasconcelos, Vítor
Tamagnini, Paula
Palavras-chave: Cyanobacteria
16S rRNA gene
Nitrogen fixation
Marine
Phylogeny
Toxins
Data: 2012
Editora: Elsevier
Citação: 2
Resumo: Cyanobacteria are important primary producers, and many are able to fix atmospheric nitrogen playing a key role in the marine environment. However, not much is known about the diversity of cyanobacteria in Portuguese marine waters. This paper describes the diversity of 60 strains isolated from benthic habitats in 9 sites (intertidal zones) on the Portuguese South and West coasts. The strains were characterized by a morphological study (light and electron microscopy) and by a molecular characterization (partial 16S rRNA, nifH, nifK, mcyA, mcyE/ndaF, sxtI genes). The morphological analyses revealed 35 morphotypes (15 genera and 16 species) belonging to 4 cyanobacterial Orders/Subsections. The dominant groups among the isolates were the Oscillatoriales. There is a broad congruence between morphological and molecular assignments. The 16S rRNA gene sequences of 9 strains have less than 97% similarity compared to the sequences in the databases, revealing novel cyanobacterial diversity. Phylogenetic analysis, based on partial 16S rRNA gene sequences showed at least 12 clusters. One-third of the isolates are potential N2-fixers, as they exhibit heterocysts or the presence of nif genes was demonstrated by PCR. Additionally, no conventional freshwater toxins genes were detected by PCR screening.
Peer review: yes
URI: http://hdl.handle.net/10400.22/6277
DOI: 10.1016/j.syapm.2011.07.003
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0723202011002372
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