Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/6222
Título: S100A6 Amyloid Fibril Formation Is Calcium-modulated and Enhances Superoxide Dismutase-1 (SOD1) Aggregation
Autor: Botelho, Hugo
S. Leal, Sónia
Cardoso, Isabel
Yanamandra, Kiran
Morozova-Roche, Ludmilla A.
Fritz, Günter
Gomes, Cláudio M.
Palavras-chave: Aggregation
Protein Aggregation
S100 Proteins
Superoxide Dismutase
Data: 2012
Editora: The American Society for Biochemistry and Molecular Biology, Inc
Resumo: S100A6 is a small EF-hand calcium- and zinc-binding protein involved in the regulation of cell proliferation and cytoskeletal dynamics. It is overexpressed in neurodegenerative disorders and a proposed marker for Amyotrophic Lateral Sclerosis (ALS). Following recent reports of amyloid formation by S100 proteins, we investigated the aggregation properties of S100A6. Computational analysis using aggregation predictors Waltz and Zyggregator revealed increased propensity within S100A6 helices HI and HIV. Subsequent analysis of Thioflavin-T binding kinetics under acidic conditions elicited a very fast process with no lag phase and extensive formation of aggregates and stacked fibrils as observed by electron microscopy. Ca2+ exerted an inhibitory effect on the aggregation kinetics, which could be reverted upon chelation. An FT-IR investigation of the early conformational changes occurring under these conditions showed that Ca2+ promotes anti-parallel β-sheet conformations that repress fibrillation. At pH 7, Ca2+ rendered the fibril formation kinetics slower: time-resolved imaging showed that fibril formation is highly suppressed, with aggregates forming instead. In the absence of metals an extensive network of fibrils is formed. S100A6 oligomers, but not fibrils, were found to be cytotoxic, decreasing cell viability by up to 40%. This effect was not observed when the aggregates were formed in the presence of Ca2+. Interestingly, native S1006 seeds SOD1 aggregation, shortening its nucleation process. This suggests a cross-talk between these two proteins involved in ALS. Overall, these results put forward novel roles for S100 proteins, whose metal-modulated aggregation propensity may be a key aspect in their physiology and function.
Peer review: yes
URI: http://hdl.handle.net/10400.22/6222
DOI: 10.1074/jbc.M112.396416
Versão do Editor: http://www.researchgate.net/profile/Claudio_Gomes3/publication/232280469_S100A6_amyloid_fibril_formation_is_calcium-modulated_and_enhances_superoxide_dismutase-1_(SOD1)_aggregation/links/0046352547e20c2bcd000000.pdf
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