Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/6174
Título: The transport of carboxylic acids and important role of the Jen1p transporter during the development of yeast colonies
Autor: Paiva, Sandra
Strachotová, Dita
Kucerová, Helena
Hlavácek, Otakar
Mota, Sandra
Casal, Margarida
Palkova, Zdena
Váchová, Libuse
Palavras-chave: ammonia production
Jen1p protein
monocarboxylic acid import
Saccharomyces cerevisiae
yeast colony development
Data: 2013
Resumo: On solid substrates, yeast colonies pass through distinct developmental phases characterized by the changes in pH of their surroundings from acidic to nearly alkaline and vice versa. At the beginning of the alkali phase colonies start to produce ammonia, which functions as a quorum-sensing molecule inducing the reprogramming of cell metabolism. Such reprogramming includes, among others, the activation of several plasma membrane transporters and is connected with colony differentiation. In the present study, we show that colony cells can use two transport mechanisms to import lactic acid: a ‘saturable’ component of the transport, which requires the presence of a functional Jen1p transporter, and a ‘non-saturable’ component (diffusion) that is independent of Jen1p. During colony development, the efficiency of both transport components changes similarly in central and outer colonial cells. Although the lactate uptake capacity of central cells gradually decreases during colony development, the lactate uptake capacity of outer cells peaks during the alkali phase and is also kept relatively high in the second acidic phase. This lactate uptake profile correlates with the localization of the Jen1p transporter to the plasma membrane of colony cells. Both lactic acid uptake mechanisms are diminished in sok2 colonies where JEN1 expression is decreased. The Sok2p transcription factor may therefore be involved in the regulation of non-saturable lactic acid uptake in yeast colonies.
Peer review: yes
URI: http://hdl.handle.net/10400.22/6174
DOI: 10.1042/BJ20120312
Versão do Editor: http://www.biochemj.org/bj/454/0551/4540551.pdf
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