Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/5324
Título: Electrochemical immunosensor for the analysis of the breast cancer biomarker HER2 ECD
Autor: Marques, Raquel Cristina Barroso
Viswanathan, Subramanian
Nouws, Henri P. A.
Delerue-Matos, Cristina
González-García, Maria Begoña
Palavras-chave: Breast cancer
Electrochemical immunosensor
Gold nanoparticles
Screen-printed carbon electrode
Data: 1-Nov-2014
Editora: Elsevier
Relatório da Série N.º: Talanta;Vol. 129
Resumo: Human epidermal growth factor receptor 2 (HER2) is a breast cancer biomarker that plays a major role in promoting breast cancer cell proliferation and malignant growth. The extracellular domain (ECD) of HER2 can be shed into the blood stream and its concentration is measurable in the serum fraction of blood. In this work an electrochemical immunosensor for the analysis of HER2 ECD in human serum samples was developed. To achieve this goal a screen-printed carbon electrode, modified with gold nanoparticles, was used as transducer surface. A sandwich immunoassay, using two monoclonal antibodies, was employed and the detection of the antibody–antigen interaction was performed through the analysis of an enzymatic reaction product by linear sweep voltammetry. Using the optimized experimental conditions the calibration curve (ip vs. log[HER2 ECD]) was established between 15 and 100 ng/mL and a limit of detection (LOD) of 4.4 ng/mL was achieved. These results indicate that the developed immunosensor could be a promising tool in breast cancer diagnostics, patient follow-up and monitoring of metastatic breast cancer since it allows quantification in a useful concentration range and has an LOD below the established cut-off value (15 ng/mL).
Peer review: yes
URI: http://hdl.handle.net/10400.22/5324
DOI: 10.1016/j.talanta.2014.06.035
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0039914014004913
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