Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/5308
Título: Modification of cell volume and proliferative capacity of Pseudokirchneriella subcapitata cells exposed to metal stress
Autor: Machado, Manuela D.
Soares, Eduardo V.
Palavras-chave: Algal growth
Biovolume
Cell division
Cell proliferation
Cell size
Metal toxicity
Data: 2014
Editora: Elsevier
Relatório da Série N.º: Aquatic Toxicology;Vol. 147
Resumo: The impact of metals (Cd, Cr, Cu and Zn) on growth, cell volume and cell division of the freshwateralga Pseudokirchneriella subcapitata exposed over a period of 72 h was investigated. The algal cells wereexposed to three nominal concentrations of each metal: low (closed to 72 h-EC10values), intermediate(closed to 72 h-EC50values) and high (upper than 72 h-EC90values). The exposure to low metal concen-trations resulted in a decrease of cell volume. On the contrary, for the highest metal concentrations anincrease of cell volume was observed; this effect was particularly notorious for Cd and less pronouncedfor Zn. Two behaviours were found when algal cells were exposed to intermediate concentrations ofmetals: Cu(II) and Cr(VI) induced a reduction of cell volume, while Cd(II) and Zn(II) provoked an oppositeeffect. The simultaneous nucleus staining and cell image analysis, allowed distinguishing three phases inP. subcapitata cell cycle: growth of mother cell; cell division, which includes two divisions of the nucleus;and, release of four autospores. The exposure of P. subcapitata cells to the highest metal concentrationsresulted in the arrest of cell growth before the first nucleus division [for Cr(VI) and Cu(II)] or after thesecond nucleus division but before the cytokinesis (release of autospores) when exposed to Cd(II). Thedifferent impact of metals on algal cell volume and cell-cycle progression, suggests that different toxic-ity mechanisms underlie the action of different metals studied. The simultaneous nucleus staining andcell image analysis, used in the present work, can be a useful tool in the analysis of the toxicity of thepollutants, in P. subcapitata, and help in the elucidation of their different modes of action.
Peer review: yes
URI: http://hdl.handle.net/10400.22/5308
DOI: 10.1016/j.aquatox.2013.11.017
ISSN: 0166-445X
Versão do Editor: http://www.sciencedirect.com/science/article/pii/S0166445X13003329
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