Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/5292
Título: Siderophore Production by Bacillus megaterium: Effect of Growth Phase and Cultural Conditions
Autor: Santos, Sofia
Neto, Isabel F. F.
Machado, Manuela D.
Soares, Helena Maria
Soares, Eduardo V.
Palavras-chave: Bacillus megaterium
Carbon source
Environmental-friendly chelating agents
Iron chelator
Siderophore production
Data: 2014
Editora: Springer
Relatório da Série N.º: Applied Biochemistry and Biotechnology;Vol. 172, Issue 1
Resumo: Siderophore production by Bacillus megaterium was detected, in an iron-deficient culture medium, during the exponential growth phase, prior to the sporulation, in the presence of glucose; these results suggested that the onset of siderophore production did not require glucose depletion and was not related with the sporulation. The siderophore production by B. megaterium was affected by the carbon source used. The growth on glycerol promoted the very high siderophore production (1,182 μmol g−1 dry weight biomass); the opposite effect was observed in the presence of mannose (251 μmol g−1 dry weight biomass). The growth in the presence of fructose, galactose, glucose, lactose, maltose or sucrose, originated similar concentrations of siderophore (546–842 μmol g−1 dry weight biomass). Aeration had a positive effect on the production of siderophore. Incubation of B. megaterium under static conditions delayed and reduced the growth and the production of siderophore, compared with the incubation in stirred conditions.
Peer review: yes
URI: http://hdl.handle.net/10400.22/5292
DOI: 10.1007/s12010-013-0562-y
Versão do Editor: http://link.springer.com/article/10.1007%2Fs12010-013-0562-y
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