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Advisor(s)
Abstract(s)
Lead is an important environmental pollutant.
The role of vacuole, in Pb detoxification, was studied using
a vacuolar protein sorting mutant strain (vps16D),
belonging to class C mutants. Cells disrupted in VPS16
gene, did not display a detectable vacuolar-like structure.
Based on the loss of cell proliferation capacity, it was
found that cells from vps16D mutant exhibited a hypersensitivity
to Pb-induced toxicity, compared to wild type
(WT) strain. The function of vacuolar H?-ATPase (VATPase),
in Pb detoxification, was evaluated using mutants
with structurally normal vacuoles but defective in subunits
of catalytic (vma1D or vma2D) or membrane domain
(vph1D or vma3D) of V-ATPase. All mutants tested,
lacking a functional V-ATPase, displayed an increased
susceptibility to Pb, comparatively to cells from WT strain.
Modification of vacuolar morphology, in Pb-exposed cells,
was visualized using a Vma2p-GFP strain. The treatment
of yeast cells with Pb originated the fusion of the medium
size vacuolar lobes into one enlarged vacuole. In conclusion,
it was found that vacuole plays an important role in
the detoxification of Pb in Saccharomyces cerevisiae; in
addition, a functional V-ATPase was required for Pb
compartmentalization.
Description
Keywords
Metals toxicity Pb detoxification Vacuolar H+-ATPase Yeast
Citation
Publisher
Springer