Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/4737
Título: Proteomic analysis of the influence of the adipocyte secretome on Glioma Gl261 cells
Autor: Costa, Joana
Orientador: Fernandes, Rúben
Sala, Carlo
Almeida, Joana
Palavras-chave: Glioma
Cancer
Adipose tissue
Obesity
Proteomics
2D
Mass spectroscopy
Data de Defesa: 2013
Editora: Instituto Politécnico do Porto. Escola Superior de Tecnologia da Saúde do Porto
Resumo: Glioma is the most frequent form of malignant brain tumor in the adults and childhood. There is a global tendency toward a higher incidence of gliomas in highly developed and industrialized countries. Simultaneously obesity is reaching epidemic proportions in such developed countries. It has been highly accepted that obesity may play an important role in the biology of several types of cancer. We have developed an in vitro method for the understanding of the influence of obesity on glioma mouse cells (Gl261). 3T3-L1 mouse pre-adipocytes were induced to the maturity. The conditioned medium was harvested and used into the Gl261 cultures. Using two-dimension electrophoresis it was analyzed the proteome content of Gl261 in the presence of conditioned medium (CGl) and in its absence (NCGl). The differently expressed spots were collected and analyzed by means of mass spectroscopy (MALDI-TOF-MS). Significantly expression pattern changes were observed in eleven proteins and enzymes. RFC1, KIF5C, ANXA2, N-RAP, RACK1 and citrate synthase were overexpressed or only present in the CGl. Contrariwise, STI1, hnRNPs and phosphoglycerate kinase 1 were significantly underexpressed in CGl. Aldose reductase and carbonic anhydrase were expressed only in NCGl. Our results show that obesity remodels the physiological and metabolic behavior of glioma cancer cells. Also, proteins found differently expressed are implicated in several signaling pathways that control matrix remodeling, proliferation, progression, migration and invasion. In general our results support the idea that obesity may increase glioma malignancy, however, some interesting paradox finding were also reported and discussed.
Peer review: yes
URI: http://hdl.handle.net/10400.22/4737
Aparece nas colecções:ESTSP - DM - Tecnologia Bioquímica em Saúde

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