Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/3601
Título: In vitro transference and molecular characterization of bla TEM genes in bacteria isolated from Portuguese ready-to-eat foods
Autor: Amador, Paula
Fernandes, Rúben
Duarte, I.
Brito, Luísa
Prudêncio, Cristina
Palavras-chave: Ready-to-eat (RTE) food
β-lactam-resistant Enterobacteriaceae
bla TEM-1 gene
Transconjugants
Data: 2011
Editora: Springer
Relatório da Série N.º: World Journal of Microbiology and Biotechnology; Vol. 27, Nº 8
Resumo: The principal aim of this study was to investigate the possibility of transference to Escherichia coli of β-lactam resistance genes found in bacteria isolated from ready-to-eat (RTE) Portuguese traditional food. From previous screenings, 128 β-lactam resistant isolates (from different types of cheese and of delicatessen meats), largely from the Enterobacteriaceae family were selected and 31.3% of them proved to transfer resistance determinants in transconjugation assays. Multiplex PCR in donor and transconjugant isolates did not detect bla CTX, bla SHV and bla OXY, but bla TEM was present in 85% of them, while two new TEMs (TEM-179 and TEM-180) were identified in two isolates. The sequencing of these amplicons showed identity between donor and transconjugant genes indicating in vitro plasmid DNA transfer. These results suggest that if there is an exchange of genes in natural conditions, the consumption of RTE foods, particularly with high levels of Enterobacteriaceae, can contribute to the spread of antibiotic resistance.
Peer review: yes
URI: http://hdl.handle.net/10400.22/3601
ISSN: 0959-3993
1573-0972
Versão do Editor: http://link.springer.com/article/10.1007%2Fs11274-010-0635-9
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