Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/3600
Título: Antibiotic resistance in enterobacteriaceae isolated from Portuguese deli meats
Autor: Amador, Paula
Fernandes, Rúben
Brito, Luísa
Prudêncio, Cristina
Data: 2011
Editora: Wiley Periodicals
Relatório da Série N.º: Journal of Food Safety; Vol. 31, Nº 1
Resumo: This study aimed to identify the presence of β-lactam-resistant bacteria in different types of Portuguese deli meats. The numbers of ampicillin resistant bacteria varied from negative in 25 g to 1.0 × 108colony-forming units/g. Within 78 randomly selected β-lactam-resistant bacteria, 24 different resistant phenotypes were found and 35.9% were multidrug resistant (MDR). The majority (87.2%) of the isolates identified belonged to the Enterobacteriaceae family. The presence of the blaTEM gene was detected in 23 out of 67 isolates (34.3%) and 16 of them presented MDR phenotypes. Four Klebsiella oxytoca isolates (6%) harbored a gene for the CTX-M/OXY-type enzyme. The direct sequencing of their purified amplicons confirmed the presence of three types of blaOXYgenes (blaOXY-1, blaOXY-2 and blaOXY-5). These results suggest that without good hygienic practices, deli meats may act as a vehicle of transfer of β-lactam-resistant bacteria to the gastrointestinal tract of consumers.
Peer review: yes
URI: http://hdl.handle.net/10400.22/3600
Versão do Editor: http://onlinelibrary.wiley.com/doi/10.1111/j.1745-4565.2010.00258.x/abstract
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