Utilize este identificador para referenciar este registo: http://hdl.handle.net/10400.22/3371
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dc.contributor.authorMachado, Manuela D.-
dc.contributor.authorSoares, Eduardo V.-
dc.date.accessioned2014-01-20T09:18:51Z-
dc.date.available2014-01-20T09:18:51Z-
dc.date.issued2013-
dc.identifierDOI 10.1007/s11270-012-1358-3-
dc.identifier.issn0049-6979-
dc.identifier.urihttp://hdl.handle.net/10400.22/3371-
dc.description.abstractThe present work describes the optimization of a short-term assay, based on the inhibition of the esterase activity of the alga Pseudokirchneriella subcapitata, in a microplate format. The optimization of the staining procedure showed that the incubation of the algal cells with 20 μmolL−1 fluorescein diacetate (FDA) for 40 min allowed discrimination between metabolic active and inactive cells. The shortterm assay was tested using Cu as toxicant. For this purpose, algal cells, in the exponential or stationary phase of growth, were exposed to the heavy metal in growing conditions. After 3 or 6 h, cells were subsequently stained with FDA, using the optimized procedure. For Cu, the 3- and 6-h EC50 values, based on the inhibition of the esterase activity of algal cells in the exponential phase of growth, were 209 and 130 μg L−1, respectively. P. subcapitata cells, in the stationary phase of growth, displayed higher effective concentration values than those observed in the exponential phase. The 3- and 6-h EC50 values for Cu, for cells in the stationary phase, were 443 and 268 μgL−1, respectively. This short-term microplate assay showed to be a rapid endpoint for testing toxicity using the alga P. subcapitata. The small volume required, the simplicity of the assay (no washing steps), and the automatic reading of the fluorescence make the assay particularly well suited for the evaluation of the toxicity of a high number of environmental samples.por
dc.language.isoengpor
dc.publisherSpringerpor
dc.relation.ispartofseriesWater, Air, & Soil Pollution; Vol. 224, Issue 1-
dc.rightsopenAccesspor
dc.subjectBioassaypor
dc.subjectFluorescein diacetate (FDA)por
dc.subjectGrowth phasepor
dc.subjectMicroplate assaypor
dc.subjectMetabolic activitypor
dc.subjectSelenastrum capricornutumpor
dc.titleOptimization of a microplate-based assay to assess esterase activity in the alga Pseudokirchneriella subcapitatapor
dc.typearticlepor
dc.peerreviewedyespor
degois.publication.firstPage1358por
degois.publication.issueIssue 1-
degois.publication.lastPage1369por
degois.publication.titleWater, Air, & Soil Pollutionpor
degois.publication.volumeVol. 224por
dc.relation.publisherversionhttp://link.springer.com/article/10.1007/s11270-012-1358-3por
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